Human Pancreatic prohormone ELISA Kit | PPY elisa kit

Human Pancreatic prohormone ELISA Kit

Cat. Num. AAA760864

• Gene Names: PPY; PP; PNP
• Reactivity: Human
• Synonyms: Pancreatic prohormone; Human Pancreatic prohormone ELISA Kit; PPY/INN/Obinepitide/PNP/PP/Obinepitide/pancreatic polypeptide (ppy)/pancreatic polypeptide/pancreatic polypeptide Y/pancreatic prohormone; PPY elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PGF2alpha. No significant cross-reactivity or interference between PGF2alpha and analogues is observed.
• Sequence Length: 95

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Competitive ELISA, Coated with Antigen
• Detection Range: 7.813-500pg/ml
• Sensitivity: <4.688pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PGF2alpha were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PGF2alpha were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PPY elisa kit

Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with PGF2alpha. During the reaction, PGF2alpha in the sample or standard competes with a fixed amount of PGF2alpha on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to PGF2alpha. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PGF2alpha in the samples is then determined by comparing the O D of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4506033
• NCBI GeneID: 5539
• NCBI Accession #: NP_002713.1
• NCBI GenBank Nucleotide #: NM_002722.4
• Molecular Weight: 18,786 Da
• NCBI Official Full Name: pancreatic prohormone isoform 1 preproprotein
• NCBI Official Synonym Full Names: pancreatic polypeptide
• NCBI Official Symbol: PPY
• NCBI Official Synonym Symbols: PP; PNP
• NCBI Protein Information: pancreatic prohormone
• UniProt Protein Name: Pancreatic prohormone
• Protein Family: Pancreatic hormone
• UniProt Gene Name: PPY
• UniProt Synonym Gene Names: PNP; PP; PH; PI
• UniProt Entry Name: PAHO_HUMAN

NCBI Description

This gene encodes a member of the neuropeptide Y (NPY) family of peptides. The encoded 95 aa preproprotein is synthesized in the pancreatic islets of Langerhans and proteolytically processed to generate two peptide products. These products include the active pancreatic hormone of 36 aa and an icosapeptide of unknown function. This hormone acts as a regulator of pancreatic and gastrointestinal functions and may be important in the regulation of food intake. Plasma level of this hormone has been shown to be reduced in conditions associated with increased food intake and elevated in anorexia nervosa. In addition, infusion of this hormone in obese rodents has shown to decrease weight gain. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]

Uniprot Description

PPY: Pancreatic hormone is synthesized in pancreatic islets of Langerhans and acts as a regulator of pancreatic and gastrointestinal functions. Belongs to the NPY family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: cytoplasm; extracellular region; extracellular space

Molecular Function: G-protein-coupled receptor binding; hormone activity; neuropeptide hormone activity; receptor binding

Biological Process: cell-cell signaling; digestion; feeding behavior; neuropeptide signaling pathway; positive regulation of defense response to virus by host; protein secretion; regulation of appetite