BCA Protein Assay Kit | BCA assay kit
BCA Protein Assay Kit
Synonyms
BCA Protein; BCA Protein Assay Kit; BCA assay kit
Principle & Procedure
plasmid purfication protocols are based on a modified alkaline lysis procedure, followed by binding of plasmid DNA to resin under appropriate low-salt and pH conditions. RNA, proteins, dyes, and low-molecular-weight impurities are removed by a medium-salt wash. Plasmid DNA is eluted in a high-salt buffer and then concentrated and desalted by isopropanol precipitation.
Determation of Yield
To determine the yield, DNA concentration should be determined by both UV spectrophotometry at 260 nm and quantitative analysis on an agarose gel. For reliable spectrophotometric DNA quantification, A260 readings should lie between 0.1 and 1.0
Note
Agarose gel analysis-We recommend removing and saving aliquots during the purification procedure. If the plasmid DNA is of low yield or quality, the samples can be analyzed by agarose gel electrophoresis to determine the stage of the purification procedure where the problem occurred.
Preparation and Storage
Spin Column CM should be stored dry and at room temperature (15-25 degree C). They can be stored for at least 2 years without showing any reduction in performance, capacity, or quality of separation. Fine Plasmid Kits should be stored at room temperature. After adding RNase A, Buffer P1 should be stored at 2-8 degree C and is stable for 6months. Other buffers and RNase A stock solution can be stored for 2 years at room temperature.
Related Product Information for BCA assay kit
Plasmid Purification Kit is designed to extract high-quality plasmid DNA for molecular biology experiments in eukaryotic transfection and in vitro expression. The kit bases on SDS-alkaline lysis method and provides a rapid operation for extraction and purification by using silica-membrane binding columns. Using the kit, plasmid DNA can be isolated in less than 1 hour with purity of A260/A280>1.86, greatly reducing the time spent on extraction and achieving high purification.
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Product Notes
The BCA (Catalog #AAA7608268) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "BCA Protein, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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