Human CLEC4M (C-type lectin domain family 4 member M) ELISA Kit | CLEC4M elisa kit

Human CLEC4M (C-type lectin domain family 4 member M) ELISA Kit

Cat. Num. AAA7608211

• Reactivity: Human
• Synonyms: CLEC4M (C-type lectin domain family 4 member M); Human CLEC4M (C-type lectin domain family 4 member M) ELISA Kit; CD209 antigen like protein 1; CD209L; CD209L1; CD299; CLEC4M; DC SIGN related protein; DC SIGN2; DC SIGNR; DCSIGNR; HP10347; L SIGN; LSIGN; CLEC4M elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CLEC4M. No significant cross-reactivity or interference between CLEC4M and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 3.125-200ng/ml
• Sensitivity: 1.875ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for est. ~6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CLEC4M elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• UniProt Accession #: Q9H2X3
• UniProt Protein Name: C-type lectin domain family 4 member M
• Protein Family: C-type lectin domain family
• UniProt Gene Name: CLEC4M
• UniProt Synonym Gene Names: CD209L; CD209L1; CD299; DC-SIGNR; DC-SIGN2; L-SIGN
• UniProt Entry Name: CLC4M_HUMAN

Uniprot Description

CLEC4M: Probable pathogen-recognition receptor involved in peripheral immune surveillance in liver. May mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens, including HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus glycoproteins, HCV E2, and human SARS coronavirus protein S. Is a receptor for ICAM3, probably by binding to mannose-like carbohydrates. Is presumably a coreceptor for the SARS coronavirus. 10 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19p13

Cellular Component: integral to plasma membrane; membrane

Molecular Function: calcium-dependent protein binding; viral receptor activity; virion binding

Biological Process: cell-cell recognition; regulation of blood coagulation; regulation of gene expression; virion attachment to host cell surface receptor; virus-host interaction