Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human lung cancer using MBS7607564 (SMARCA4 antibody) at dilution of 1:100)
Rabbit anti-Human, Mouse SMARCA4 Polyclonal Antibody | anti-SMARCA4 antibody
Anti-SMARCA4 Antibody
Gene Names
SMARCA4; BRG1; CSS4; SNF2; SWI2; MRD16; RTPS2; BAF190; SNF2L4; SNF2LB; hSNF2b; BAF190A; SNF2-beta
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
>=95% as determined by SDS-PAGEImmunogen affinity purified
Synonyms
SMARCA4; Polyclonal Antibody; Anti-SMARCA4 Antibody; ATP dependent helicase SMARCA4; BAF190; BAF190A; BRG1; BRG1 associated factor 190A; FLJ39786; hSNF2b; Protein brahma homolog 1; Protein BRG 1; SNF2; SNF2 BETA; SNF2B; SNF2L4; SNF2LB; SWI2; Transcription activator BRG1; anti-SMARCA4 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>=95% as determined by SDS-PAGE
Immunogen affinity purified
Immunogen affinity purified
Form/Format
Liquid; PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Sequence Length
1647
Applicable Applications for anti-SMARCA4 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
Preparation and Storage
Store at -20 degree C for 24 months. Avoid repeated freeze/thaw cycles.
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human lung cancer using MBS7607564 (SMARCA4 antibody) at dilution of 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human lung cancer using MBS7607564 (SMARCA4 antibody) at dilution of 1:100)
Western Blot (WB)
(HeLa cells were subjected to SDS PAGE followed by western blot with MBS7607564 (SMARCA4 antibody) at dilution of 1:1000)
Western Blot (WB)
(HeLa cells were subjected to SDS PAGE followed by western blot with MBS7607564 (SMARCA4 antibody) at dilution of 1:1000)
Related Product Information for anti-SMARCA4 antibody
SMARCA4, also named as BAF190A, BRG1, SNF2B and SNF2L4, belongs to the SNF2/RAD54 helicase family. SMARCA4 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. It is a component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor(VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
Product Categories/Family for anti-SMARCA4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed MW: 185kDa
NCBI Official Full Name
transcription activator BRG1 isoform B
NCBI Official Synonym Full Names
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
NCBI Official Symbol
SMARCA4
NCBI Official Synonym Symbols
BRG1; CSS4; SNF2; SWI2; MRD16; RTPS2; BAF190; SNF2L4; SNF2LB; hSNF2b; BAF190A; SNF2-beta
NCBI Protein Information
transcription activator BRG1
UniProt Protein Name
Transcription activator BRG1
Protein Family
UniProt Gene Name
SMARCA4
UniProt Synonym Gene Names
BAF190A; BRG1; SNF2B; SNF2L4; BAF190A
UniProt Entry Name
SMCA4_HUMAN
NCBI Description
The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]
Research Articles on SMARCA4
Similar Products
Product Notes
The SMARCA4 smarca4 (Catalog #AAA7607564) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SMARCA4 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SMARCA4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the SMARCA4 smarca4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMARCA4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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