Mouse PDI Antibody | anti-PDI antibody

Anti-PDI Antibody

Cat. Num. AAA7607354

• Gene Names: P4HB; DSI; GIT; PDI; PHDB; PDIA1; PO4DB; PO4HB; PROHB; CLCRP1; ERBA2L; P4Hbeta
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: >=95% as determined by SDS-PAGE; Protein A+G Purification
• Synonyms: PDI; Anti-PDI Antibody; DSI; ERBA2L; GIT; P4HB; P4Hbeta; p55; PDIA1; PHDB; PO4DB; PO4HB; PROHB; Protein disulfide isomerase; anti-PDI antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Isotype: IgG2b
• Purity/Purification: >=95% as determined by SDS-PAGE; Protein A+G Purification
• Form/Format: Liquid; PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Sequence Length: 508

• Applicable Applications for anti-PDI antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:10000-1:100000; IHC: 1:100-1:400; IF: 1:50-1:500
• Immunogen: Prolyl 4-hydroxylase, beta polypeptide
• Preparation and Storage: Store at -20 degree C for 24 months. Avoid repeated freeze/thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver tissue slide using MBS7607354 (PDI Antibody) at dilution of 1:200)

Immunofluorescence (IF)

(Immunofluorescent analysis of (-20 degree C Ethanol) fixed HeLa cells using MBS7607354 (PDI antibody) at dilution of 1:100 and Alexa Fluor 488-congugated Goat Anti-Mouse IgG (H+L))

Western Blot (WB)

(HEK-293 cells were subjected to SDS PAGE followed by western blot with MBS7607354 (PDI antibody) at dilution of 1:50000)

Related Product Information for anti-PDI antibody

PDIA1(Protein disulfide-isomerase) is also named as ERBA2L, PDI, P4HB, PO4DB. It is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. In some cell types, it seems to be secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources.It can exsit as homodimer and monomers and homotetramers may also occur(PMID:12095988).

NCBI and Uniprot Product Information

• NCBI GI #: 20070125
• NCBI GeneID: 5034
• NCBI Accession #: NP_000909.2
• NCBI GenBank Nucleotide #: NM_000918.4
• UniProt Accession #: P07237
• Molecular Weight: 120 kDa
• NCBI Official Full Name: protein disulfide-isomerase
• NCBI Official Synonym Full Names: prolyl 4-hydroxylase subunit beta
• NCBI Official Symbol: P4HB
• NCBI Official Synonym Symbols: DSI; GIT; PDI; PHDB; PDIA1; PO4DB; PO4HB; PROHB; CLCRP1; ERBA2L; P4Hbeta
• NCBI Protein Information: protein disulfide-isomerase
• UniProt Protein Name: Protein disulfide-isomerase
• UniProt Gene Name: P4HB
• UniProt Synonym Gene Names: ERBA2L; PDI; PDIA1; PO4DB; PDI
• UniProt Entry Name: PDIA1_HUMAN

NCBI Description

This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex. [provided by RefSeq, Jul 2008]