Human LAMTOR1 (Ragulator complex protein LAMTOR1) ELISA Kit | LAMTOR1 elisa kit

Human LAMTOR1 (Ragulator complex protein LAMTOR1) ELISA Kit

Cat. Num. AAA7606237

• Gene Names: LAMTOR1; p18; PDRO; C11orf59; p27RF-Rho; Ragulator1
• Reactivity: Human
• Synonyms: LAMTOR1 (Ragulator complex protein LAMTOR1); Human LAMTOR1 (Ragulator complex protein LAMTOR1) ELISA Kit; LAMTOR1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of LAMTOR1. No significant cross-reactivity or interference between LAMTOR1 and analogues was observed.
• Sequence Length: 161

• Samples: Cell lysates, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LAMTOR1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 125863645
• NCBI GeneID: 55004
• UniProt Accession #: Q6IAA8; Q8WZ09; Q9NWT0
• Molecular Weight: 17,745 Da
• NCBI Official Full Name: Ragulator complex protein LAMTOR1
• NCBI Official Synonym Full Names: late endosomal/lysosomal adaptor, MAPK and MTOR activator 1
• NCBI Official Symbol: LAMTOR1
• NCBI Official Synonym Symbols: p18; PDRO; C11orf59; p27RF-Rho; Ragulator1
• NCBI Protein Information: ragulator complex protein LAMTOR1
• UniProt Protein Name: Ragulator complex protein LAMTOR1
• Protein Family: Ragulator complex protein
• UniProt Gene Name: LAMTOR1
• UniProt Synonym Gene Names: C11orf59; PDRO; p27RF-Rho

Uniprot Description

LAMTOR1: Regulator of the TOR pathway, a signaling cascade that promotes cell growth in response to growth factors, energy levels, and amino acids. As part of the Ragulator complex, recruits the Rag GTPases and the mTORC1 complex to lysosomes, a key step in activation of the TOR signaling cascade by amino acids. Directly responsible for anchoring the Ragulator complex to membranes. Also required for late endosomes/lysosomes biogenesis it may regulate both the recycling of receptors through endosomes and the MAPK signaling pathway through recruitment of some of its components to late endosomes. May be involved in cholesterol homeostasis regulating LDL uptake and cholesterol release from late endosomes/lysosomes. May also play a role in RHOA activation. Part of the Ragulator complex composed of LAMTOR1, LAMTOR2 and LAMTOR3. Interacts with LAMTOR2 and LAMTOR3; the interaction is direct. The Ragulator complex interacts with both the mTORC1 complex and heterodimers of the Rag GTPases RRAGA, RRAGB, RRAGC and RRAGD. Interacts with RRAGB and RRAGD; the interaction is direct indicating that it probably constitutes the main RAG-interacting subunit of the Ragulator complex. Interacts with MMP14. Interacts with CDKN1B; prevents the interaction of CDKN1B with RHOA leaving RHOA in a form accessible to activation by ARHGEF2.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 11q13.4

Cellular Component: late endosome membrane; lipid raft; lysosomal membrane; lysosome; plasma membrane

Molecular Function: GTPase binding; guanyl-nucleotide exchange factor activity; protein binding; protein complex scaffold

Biological Process: cell cycle arrest; cell growth; cholesterol homeostasis; endosome localization; endosome organization and biogenesis; lysosome localization; lysosome organization and biogenesis; macroautophagy; neutrophil degranulation; positive regulation of MAPKKK cascade; positive regulation of TOR signaling pathway; regulation of receptor recycling