Typical Testing Data/Standard Curve (for reference only)
Human CALCRL (calcitonin receptor-like receptor) ELISA Kit | CALCRL elisa kit
Human CALCRL (calcitonin receptor-like receptor) ELISA Kit
Gene Names
CALCRL; CRLR; CGRPR
Reactivity
Human
Synonyms
CALCRL (calcitonin receptor-like receptor); Human CALCRL (calcitonin receptor-like receptor) ELISA Kit; CALCRL elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of CAF. No significant cross-reactivity or interference between CAF and analogues was observed.
Sequence Length
461
Samples
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
Assay Type
Sandwich
Detection Range
46.875-3000pg/ml
Sensitivity
<28.125pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CAF were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CAF were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CALCRL elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-CAF antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-CAF antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CAF amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CAF can be calculated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
52,929 Da
NCBI Official Full Name
Calcitonin gene-related peptide type 1 receptor
NCBI Official Synonym Full Names
calcitonin receptor like receptor
NCBI Official Symbol
CALCRL
NCBI Official Synonym Symbols
CRLR; CGRPR
NCBI Protein Information
calcitonin gene-related peptide type 1 receptor
UniProt Protein Name
Calcitonin gene-related peptide type 1 receptor
Protein Family
UniProt Gene Name
CALCRL
UniProt Synonym Gene Names
CGRPR; CGRP type 1 receptor
Uniprot Description
CALCRL: Receptor for calcitonin-gene-related peptide (CGRP) together with RAMP1 and receptor for adrenomedullin together with RAMP3. Receptor for adrenomedullin together with RAMP2. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Belongs to the G-protein coupled receptor 2 family.
Protein type: Cell cycle regulation; GPCR, family 2; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR
Chromosomal Location of Human Ortholog: 2q32.1
Cellular Component: endoplasmic reticulum; endosome; integral to plasma membrane; lysosome; plasma membrane
Molecular Function: adrenomedullin receptor activity; G-protein coupled receptor activity; protein binding; protein transporter activity
Biological Process: angiogenesis; calcium ion transport; cAMP biosynthetic process; G-protein signaling, coupled to cyclic nucleotide second messenger; positive regulation of cAMP biosynthetic process; protein transport; receptor internalization
Research Articles on CALCRL
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Product Notes
The Human CALCRL calcrl (Catalog #AAA7606047) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7606047 ELISA Kit recognizes Human CALCRL. It is sometimes possible for the material contained within the vial of "CALCRL (calcitonin receptor-like receptor), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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