Human Eukaryotic translation initiation factor 3 subunit A ELISA Kit | EIF3A elisa kit

Human Eukaryotic translation initiation factor 3 subunit A ELISA Kit

Cat. Num. AAA760298

• Gene Names: EIF3A; EIF3; P167; p180; p185; TIF32; EIF3S10; eIF3-p170; eIF3-theta
• Reactivity: Human
• Synonyms: Eukaryotic translation initiation factor 3 subunit A; Human Eukaryotic translation initiation factor 3 subunit A ELISA Kit; EIF3A/Eukaryotic translation initiation factor 3 subunit A/eIF3a/eIF3 p185/eIF3 p180/eIF3 p167/Eukaryotic translation initiation factor 3 subunit 10/eIF-3-theta/EIF3S10; EIF3A elisa kit

• Reactivity: Human
• Sequence Length: 1382

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for EIF3A elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human EIF3A antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human EIF3A antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human EIF3A amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human EIF3A can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4503509
• NCBI GeneID: 8661
• NCBI Accession #: NP_003741.1
• NCBI GenBank Nucleotide #: NM_003750.2
• Molecular Weight: 162,636 Da
• NCBI Official Full Name: eukaryotic translation initiation factor 3 subunit A
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 3 subunit A
• NCBI Official Symbol: EIF3A
• NCBI Official Synonym Symbols: EIF3; P167; p180; p185; TIF32; EIF3S10; eIF3-p170; eIF3-theta
• NCBI Protein Information: eukaryotic translation initiation factor 3 subunit A
• UniProt Protein Name: Eukaryotic translation initiation factor 3 subunit A
• Protein Family: Eukaryotic translation initiation factor
• UniProt Gene Name: EIF3A
• UniProt Synonym Gene Names: eIF3a
• UniProt Entry Name: EIF3A_HUMAN

Uniprot Description

eIF3-theta: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Interacts with EIF4G1. Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3L and EIF3K. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Also interacts with KRT7 and PIWIL2. Belongs to the eIF-3 subunit A family.

Protein type: RNA-binding; Translation; Translation initiation

Chromosomal Location of Human Ortholog: 10q26

Cellular Component: cytoplasm; cytosol; eukaryotic translation initiation factor 3 complex; membrane; nucleolus; nucleus

Molecular Function: protein binding; structural molecule activity; translation initiation factor activity

Biological Process: cellular protein metabolic process; formation of translation initiation complex; formation of translation preinitiation complex; gene expression; regulation of translational initiation; translation; translational initiation