Rabbit anti-Human LRWD1 Polyclonal Antibody | anti-LRWD1 antibody

LRWD1 Rabbit Polyclonal

Cat. Num. AAA7601100

• Gene Names: LRWD1; ORCA; CENP-33
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: LRWD1; Polyclonal Antibody; LRWD1 Rabbit Polyclonal; anti-LRWD1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 495

• Applicable Applications for anti-LRWD1 antibody: ELISA (EIA), Western Blot (WB)
• Immunogen: Leucine-rich repeats and WD repeat domain containing 1
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Western Blot (WB)

(human testis tissue were subjected to SDS PAGE followed by western blot with MBS7601100 (LRWD1 antibody) at dilution of 1:100)

NCBI and Uniprot Product Information

• NCBI GI #: 959241447
• NCBI GeneID: 222229
• NCBI Accession #: NP_001304650.1
• NCBI GenBank Nucleotide #: NM_001317721.1
• Molecular Weight: 70,861 Da
• NCBI Official Full Name: leucine-rich repeat and WD repeat-containing protein 1 isoform 2
• NCBI Official Synonym Full Names: leucine rich repeats and WD repeat domain containing 1
• NCBI Official Symbol: LRWD1
• NCBI Official Synonym Symbols: ORCA; CENP-33
• NCBI Protein Information: leucine-rich repeat and WD repeat-containing protein 1
• UniProt Protein Name: Leucine-rich repeat and WD repeat-containing protein 1
• Protein Family: Leucine-rich repeat and WD repeat-containing protein
• UniProt Gene Name: LRWD1
• UniProt Synonym Gene Names: CENP33; ORCA; CENP-33; ORC-associated protein; ORCA
• UniProt Entry Name: LRWD1_HUMAN

NCBI Description

The protein encoded by this gene interacts with components of the origin recognition complex (ORC) and regulates the formation of the prereplicative complex. The encoded protein stabilizes the ORC and therefore aids in DNA replication. This protein is required for the G1/S phase transition of the cell cycle. In addition, the encoded protein binds to trimethylated histone H3 in heterochromatin and recruits the ORC and lysine methyltransferases, which help maintain the repressive heterochromatic state. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2015]

Uniprot Description

LRWD1: Associated component of the origin recognition complex (ORC) required to recruit and stabilize the ORC complex to chromatin. Probably required for the association of ORC on chromatin during G1 to establish pre-replication complex (preRC) and to heterochromatic sites in post-replicated cells. Binds a combination of DNA and histone methylation repressive marks on heterochromatin: binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3 in a cooperative manner with DNA methylation. It is unclear whether it recongnizes and binds these repressive marks by itself or needs additional factors. Belongs to the LRWD1 family.

Protein type: DNA-binding; Histone-binding; DNA replication

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: centric heterochromatin; cytoplasm; microtubule organizing center; nuclear origin of replication recognition complex; nucleus

Molecular Function: chromatin binding; methyl-CpG binding; methylated histone residue binding; protein binding

Biological Process: chromatin modification; DNA replication initiation; establishment and/or maintenance of chromatin architecture