Mouse Leukotriene B4 receptor 1 ELISA Kit | Ltb4r elisa kit

Mouse Leukotriene B4 receptor 1 ELISA Kit

Cat. Num. AAA760097

• Gene Names: LTB4R; BLT1; BLTR; P2Y7; GPR16; LTBR1; P2RY7; CMKRL1; LTB4R1
• Reactivity: Mouse
• Synonyms: Leukotriene B4 receptor 1; Mouse Leukotriene B4 receptor 1 ELISA Kit; Ltb4r/LTB4-R 1/LTB4-R1//P2Y purinoceptor 7/P2Y7/Chemoattractant receptor-like 1/G-protein coupled receptor 16/BLT1/LTB4R1/chemokine receptor-like 1/CMKRL1/leukotriene B4 receptor/LTBR1/purinergic receptor P2Y; G-protein coupled; 7; Ltb4r elisa kit

• Reactivity: Mouse
• Sequence Length: 352

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Ltb4r elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse Ltb4r antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Ltb4r antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Ltb4r amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Ltb4r can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 221218998
• NCBI GeneID: 1241
• NCBI Accession #: NP_001137391.1
• NCBI GenBank Nucleotide #: NM_001143919.2
• Molecular Weight: 37,557 Da
• NCBI Official Full Name: leukotriene B4 receptor 1
• NCBI Official Synonym Full Names: leukotriene B4 receptor
• NCBI Official Symbol: LTB4R
• NCBI Official Synonym Symbols: BLT1; BLTR; P2Y7; GPR16; LTBR1; P2RY7; CMKRL1; LTB4R1
• NCBI Protein Information: leukotriene B4 receptor 1
• UniProt Protein Name: Leukotriene B4 receptor 1
• Protein Family: Leukotriene B4 receptor
• UniProt Gene Name: LTB4R
• UniProt Synonym Gene Names: BLT; BLT1; BLTR; CMKRL1; GPR16; P2RY7; LTB4-R 1; LTB4-R1; P2Y7
• UniProt Entry Name: LT4R1_HUMAN

Uniprot Description

LTB4R: Receptor for extracellular ATP > UTP and ADP. The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system. May be the cardiac P2Y receptor involved in the regulation of cardiac muscle contraction through modulation of L-type calcium currents. Is a receptor for leukotriene B4, a potent chemoattractant involved in inflammation and immune response. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 14q11.2-q12

Cellular Component: integral to plasma membrane; lipid raft; plasma membrane

Molecular Function: leukotriene B4 receptor activity; leukotriene receptor activity; nucleotide binding; peptide receptor activity, G-protein coupled

Biological Process: cell motility; G-protein coupled receptor protein signaling pathway; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); immune response; inflammatory response; muscle contraction; neuropeptide signaling pathway