Mouse anti-Human IFITM3 Monoclonal Antibody | anti-IFITM3 antibody

IFITM3 Mouse Monoclonal

Cat. Num. AAA7600378

• Gene Names: IFITM3; 1-8U; IP15; DSPA2b
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunofluorescence, Immunohistochemistry, Flow Cytometry
• Purity: >=95% as determined by SDS-PAGE; Protein A+G purification
• Synonyms: IFITM3; Monoclonal Antibody; IFITM3 Mouse Monoclonal; 1 8U; IP15; anti-IFITM3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: Human; other species are not tested. Please decide the specificity by homology
• Purity/Purification: >=95% as determined by SDS-PAGE; Protein A+G purification
• Form/Format: Liquid
• Sequence Length: 133

• Applicable Applications for anti-IFITM3 antibody: ELISA, WB, IF, IHC, FC
• Application Notes: WB : 1:500-1:2000; IHC : 1:20-1:200; IF : 1:10-1:100
• Immunogen: IFIT2/3 fusion protein.
• Calculated MW: 14 kDa
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human lung cancer using MBS7600378 (IFITM3 antibody) at dilution of 1:50)

Immunofluorescence (IF)

(Immunofluorescent analysis of HepG2 cells, using IFITM3 antibody MBS7600378 at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red))

Western Blot (WB)

(HeLa cells were subjected to SDS PAGE followed by western blot with MBS7600378 (IFITM3 antibody) at dilution of 1:1000)

Related Product Information for anti-IFITM3 antibody

IFN-induced antiviral protein which disrupts intracellular cholesterol homeostasis.Inhibits the entry of viruses to the host cell cytoplasm by preventing viral fusion with cholesterol depleted endosomes. May inactivate new enveloped viruses which buds out of the infected cell,by letting them go out with a cholesterol depleted membrane.Active against multiple viruses, including influenza A virus, SARS coronavirus(SARS-CoV).Marbug virus(MARV) and Ebola virus(EBOV),Dengue virus(DNV),West Nile virus(WNV),human immunodeficiency virus type 1(HIV-1) and vesicular stomatitis virus (VSV).Can inhibit influenza virus hemagglutinin protein-mediated viral entry, MARV and EBOV GP1,2-mediated viral entry, SARS-CoV S protein-mediated viral entry and VSV G protein-mediated viral entry. Plays a critical role in the structural stability and function of vacuolar ATPase(v-ATPase).Establiches physical contact with the v-ATPase of endosomes which is critical for proper clathrin localization and is also required for the function of the v-ATPase to lowew the pH in phagocytic endosomes thus establishing an antiviral state. This antibody recognizes both IFITM2 and IFTIM3.

NCBI and Uniprot Product Information

• NCBI GI #: 381148134
• NCBI GeneID: 10410
• UniProt Accession #: Q01628; Q53Y76; Q96HK8; Q96J15
• NCBI Official Full Name: IFITM3
• NCBI Official Synonym Full Names: interferon induced transmembrane protein 3
• NCBI Official Symbol: IFITM3
• NCBI Official Synonym Symbols: 1-8U; IP15; DSPA2b
• NCBI Protein Information: interferon-induced transmembrane protein 3
• UniProt Protein Name: Interferon-induced transmembrane protein 3
• Protein Family: Interferon-induced transmembrane protein
• UniProt Gene Name: IFITM3
• UniProt Synonym Gene Names: DSPA2b
• UniProt Entry Name: IFM3_HUMAN

NCBI Description

The protein encoded by this gene is an interferon-induced membrane protein that helps confer immunity to influenza A H1N1 virus, West Nile virus, and dengue virus. Two transcript variants, only one of them protein-coding, have been found for this gene. Another variant encoding an N-terminally truncated isoform has been reported, but the full-length nature of this variant has not been determined. [provided by RefSeq, May 2012]

Uniprot Description

IFITM3: IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus (WNV), by inhibiting the early step(s) of replication. Interacts with SPP1; the interaction reduces OPN expression. By IFN-alpha and IFNG/IFN-gamma. Belongs to the CD225/Dispanin family.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: integral to membrane; late endosome membrane; lysosomal membrane; plasma membrane

Molecular Function: protein binding

Biological Process: defense response to virus; immune response; negative regulation of viral genome replication; negative regulation of viral transcription; negative regulation of virion penetration into host cell; response to virus

Disease: Influenza, Severe, Susceptibility To