Bovine Collagen Type XI Alpha 1 ELISA Kit | COL11a1 elisa kit

Bovine Collagen Type XI Alpha 1 ELISA Kit

Cat. Num. AAA755603

• Gene Names: COL11A1; STL2; COLL6; CO11A1
• Reactivity: Bovine
• Synonyms: Collagen Type XI Alpha 1; Bovine Collagen Type XI Alpha 1 ELISA Kit; COL11a1 elisa kit

• Reactivity: Bovine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for COL11a1 elisa kit

Principle of the assay: COL11a1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-COL11a1 antibody and an COL11a1-HRP conjugate. The assay sample and buffer are incubated together with COL11a1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the COL11a1 concentration since COL11a1 from samples and COL11a1-HRP conjugate compete for the anti-COL11a1 antibody binding site. Since the number of sites is limited, as more sites are occupied by COL11a1 from the sample, fewer sites are left to bind COL11a1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COL11a1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for COL11a1 elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 299523257
• NCBI GeneID: 1301
• NCBI Accession #: NP_001177638.1
• NCBI GenBank Nucleotide #: NM_001190709.1
• Molecular Weight: 167,752 Da
• NCBI Official Full Name: collagen alpha-1(XI) chain isoform E preproprotein
• NCBI Official Synonym Full Names: collagen, type XI, alpha 1
• NCBI Official Symbol: COL11A1
• NCBI Official Synonym Symbols: STL2; COLL6; CO11A1
• NCBI Protein Information: collagen alpha-1(XI) chain; collagen alpha-1(XI) chain; collagen XI, alpha-1 polypeptide
• UniProt Protein Name: Collagen alpha-1(XI) chain
• Protein Family: Collagen
• UniProt Gene Name: COL11A1
• UniProt Synonym Gene Names: COLL6
• UniProt Entry Name: COBA1_HUMAN

NCBI Description

This gene encodes one of the two alpha chains of type XI collagen, a minor fibrillar collagen. Type XI collagen is a heterotrimer but the third alpha chain is a post-translationally modified alpha 1 type II chain. Mutations in this gene are associated with type II Stickler syndrome and with Marshall syndrome. A single-nucleotide polymorphism in this gene is also associated with susceptibility to lumbar disc herniation. Multiple transcript variants have been identified for this gene. [provided by RefSeq, Nov 2009]

Uniprot Description

COL11A1: May play an important role in fibrillogenesis by controlling lateral growth of collagen II fibrils. Defects in COL11A1 are the cause of Stickler syndrome type 2 (STL2); also known as Stickler syndrome vitreous type 2. STL2 is an autosomal dominant form of Stickler syndrome, an inherited disorder that associates ocular signs with more or less complete forms of Pierre Robin sequence, bone disorders and sensorineural deafness. Ocular disorders may include juvenile cataract, myopia, strabismus, vitreoretinal or chorioretinal degeneration, retinal detachment, and chronic uveitis. Robin sequence includes an opening in the roof of the mouth (a cleft palate), a large tongue (macroglossia), and a small lower jaw (micrognathia). Bones are affected by slight platyspondylisis and large, often defective epiphyses. Juvenile joint laxity is followed by early signs of arthrosis. The degree of hearing loss varies among affected individuals and may become more severe over time. Syndrome expressivity is variable. Defects in COL11A1 are the cause of Marshall syndrome (MRSHS). It is an autosomal dominant disorder characterized by ocular abnormalities, deafness, craniofacial anomalies, and anhidrotic ectodermal dysplasia. Clinical features include short stature; flat or retruded midface with short, depressed nose, flat nasal bridge and anteverted nares; cleft palate with or without the Pierre Robin sequence; appearance of large eyes with ocular hypertelorism; cataracts, either congenital or juvenile; esotropia; high myopia; sensorineural hearing loss; spondyloepiphyseal abnormalities; calcification of the falx cerebri; ectodermal abnormalities, including defects in sweating and dental structures. Defects in COL11A1 are the cause of fibrochondrogenesis type 1 (FBCG1). A severe short-limbed skeletal dysplasia characterized by broad long-bone metaphyses, pear-shaped vertebral bodies, and characteristic morphology of the growth plate, in which the chondrocytes have a fibroblastic appearance and there are regions of fibrous cartilage extracellular matrix. Clinical features include a flat midface with a small nose and anteverted nares, significant shortening of all limb segments but relatively normal hands and feet, and a small bell-shaped thorax with a protuberant abdomen. Belongs to the fibrillar collagen family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Extracellular matrix; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1p21

Cellular Component: collagen type XI; endoplasmic reticulum lumen; extracellular region

Molecular Function: protein binding, bridging; extracellular matrix binding; metal ion binding; extracellular matrix structural constituent

Biological Process: extracellular matrix disassembly; collagen catabolic process; proteoglycan metabolic process; ossification; extracellular matrix organization and biogenesis; inner ear morphogenesis; sensory perception of sound; collagen fibril organization; visual perception; ventricular cardiac muscle morphogenesis; chondrocyte development; detection of mechanical stimulus involved in sensory perception of sound; cartilage condensation; embryonic skeletal morphogenesis

Disease: Intervertebral Disc Disease; Stickler Syndrome, Type Ii; Fibrochondrogenesis 1; Marshall Syndrome