Bovine Growth Factor Receptor Bound Protein 7 ELISA Kit | Grb7 elisa kit

Bovine Growth Factor Receptor Bound Protein 7 ELISA Kit

Cat. Num. AAA755205

• Reactivity: Bovine
• Synonyms: Growth Factor Receptor Bound Protein 7; Bovine Growth Factor Receptor Bound Protein 7 ELISA Kit; Grb7 elisa kit

• Reactivity: Bovine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Intended Uses: This Grb7 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine Grb7. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This Grb7 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine Grb7. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Grb7 elisa kit

Principle of the Assay: Grb7 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Grb7 antibody and an Grb7-HRP conjugate. The assay sample and buffer are incubated together with Grb7-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Grb7 concentration since Grb7 from samples and Grb7-HRP conjugate compete for the anti-Grb7 antibody binding site. Since the number of sites is limited, as more sites are occupied by Grb7 from the sample, fewer sites are left to bind Grb7-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Grb7 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 16306816
• NCBI GeneID: 2886
• Molecular Weight: 55,920 Da
• NCBI Official Full Name: Growth factor receptor-bound protein 7
• NCBI Official Synonym Full Names: growth factor receptor-bound protein 7
• NCBI Official Symbol: GRB7
• NCBI Protein Information: growth factor receptor-bound protein 7; B47; GRB7 adapter protein; epidermal growth factor receptor GRB-7
• UniProt Protein Name: Growth factor receptor-bound protein 7
• Protein Family: Growth factor receptor-bound protein
• UniProt Gene Name: GRB7
• UniProt Entry Name: GRB7_HUMAN

NCBI Description

The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with epidermal growth factor receptor (EGFR) and ephrin receptors. The protein plays a role in the integrin signaling pathway and cell migration by binding with focal adhesion kinase (FAK). Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2011]

Uniprot Description

GRB7: Adapter protein that interacts with the cytoplasmic domain of numerous receptor kinases and modulates down-stream signaling. Promotes activation of down-stream protein kinases, including STAT3, AKT1, MAPK1 and/or MAPK3. Promotes activation of HRAS. Plays a role in signal transduction in response to EGF. Plays a role in the regulation of cell proliferation and cell migration. Plays a role in the assembly and stability of RNA stress granules. Binds to the 5'UTR of target mRNA molecules and represses translation of target mRNA species, when not phosphorylated. Phosphorylation impairs RNA binding and promotes stress granule disassembly during recovery after cellular stress. Homodimer. Interacts (via SH2 domain) with EGFR, ERBB2, ERBB3 (when phosphorylated), ERBB4 (when phosphorylated), EPHB1, INSR, FGFR1, PDGFRA (tyrosine phosphorylated) and PDGFRB (tyrosine phosphorylated). Interacts with SHC1. Interacts with RND1. Interacts (when tyrosine phosphorylated) with FHL2 and HAX1. Interacts (via SH2 domain) with RET and PTK2/FAK1. Interacts (when not phosphorylated) with ELAVL1. In stressed cells, but not in normal cells, part of a complex that contains at least GRB7, PTK2/FAK1, STAU1, ELAVL1 and TIA1. Interacts (via SH2 domain) with KIT (phosphorylated). Interacts (via SH2 domain) with TEK/TIE2 (tyrosine phosphorylated). Belongs to the GRB7/10/14 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: cell projection; focal adhesion; stress granule; plasma membrane; cytosol

Molecular Function: identical protein binding; protein binding; SH3/SH2 adaptor activity; RNA binding; protein kinase binding; phosphoinositide binding

Biological Process: epidermal growth factor receptor signaling pathway; stress granule assembly; positive regulation of signal transduction; negative regulation of translation; blood coagulation; leukocyte migration; positive regulation of cell migration