Typical Testing Data/Standard Curve (for reference only)
Goat Growth-regulated oncogeneY/melanoma growth stimulating activity ELISA Kit | BGOy / MGSA elisa kit
Goat Growth-regulated oncogeneY/melanoma growth stimulating activity ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: BGOy/MGSA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BGOy/MGSA antibody and an BGOy/MGSA-HRP conjugate. The assay sample and buffer are incubated together with BGOy/MGSA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BGOy/MGSA concentration since BGOy/MGSA from samples and BGOy/MGSA-HRP conjugate compete for the anti-BGOy/MGSA antibody binding site. Since the number of sites is limited, as more sites are occupied by BGOy/MGSA from the sample, fewer sites are left to bind BGOy/MGSA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BGOy/MGSA concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the CXC subfamily of chemokines. The encoded protein is a secreted growth factor that signal through the G-protein coupled receptor, CXC receptor 2. This protein plays a role in inflammation and as a chemoattractant for neutrophils. Aberrant expression this protein is associated with the growth and progression of certain tumors. A naturally occurring processed form of this protein has increased chemotactic activity. Alternate splicing results in coding and non-coding variants of this gene. A pseudogene of this gene is found on chromosome 4. [provided by RefSeq, Jan 2012]
Uniprot Description
CXCL1: Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO- alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity. Belongs to the intercrine alpha (chemokine CxC) family.
Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 4q21
Cellular Component: extracellular space; extracellular region
Molecular Function: growth factor activity; chemokine activity; CXCR chemokine receptor binding; enzyme activator activity; receptor binding
Biological Process: positive regulation of catalytic activity; negative regulation of cell proliferation; cell proliferation; nervous system development; G-protein coupled receptor protein signaling pathway; response to lipopolysaccharide; positive regulation of leukocyte chemotaxis; immune response; chemotaxis; signal transduction; actin cytoskeleton organization and biogenesis; inflammatory response
Research Articles on BGOy / MGSA
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Product Notes
The Goat BGOy / MGSA cxcl1 (Catalog #AAA755111) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA755111 ELISA Kit recognizes Goat BGOy / MGSA. It is sometimes possible for the material contained within the vial of "Growth-regulated oncogeneY/melanoma growth stimulating activity, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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