Typical Testing Data/Standard Curve (for reference only)
Sheep Ribonuclease T2 ELISA Kit | RNASET2 elisa kit
Sheep Ribonuclease T2 ELISA Kit
Gene Names
Rnaset2a; C79750; Rnaset2; RNASE6PL; 0610007O07Rik; 4833423A10Rik; 4930532K22Rik
Reactivity
Sheep
Synonyms
Ribonuclease T2; Sheep Ribonuclease T2 ELISA Kit; RNASET2 elisa kit
Reactivity
Sheep
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for RNASET2 elisa kit
Intended Uses: This RNASET2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep RNASET2. This ELISA kit for research use only!
Principle of the Assay: RNASET2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RNASET2 antibody and an RNASET2-HRP conjugate. The assay sample and buffer are incubated together with RNASET2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RNASET2 concentration since RNASET2 from samples and RNASET2-HRP conjugate compete for the anti-RNASET2 antibody binding site. Since the number of sites is limited, as more sites are occupied by RNASET2 from the sample, fewer sites are left to bind RNASET2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RNASET2 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: RNASET2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RNASET2 antibody and an RNASET2-HRP conjugate. The assay sample and buffer are incubated together with RNASET2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RNASET2 concentration since RNASET2 from samples and RNASET2-HRP conjugate compete for the anti-RNASET2 antibody binding site. Since the number of sites is limited, as more sites are occupied by RNASET2 from the sample, fewer sites are left to bind RNASET2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RNASET2 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for RNASET2 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
29,609 Da
NCBI Official Full Name
ribonuclease T2
NCBI Official Synonym Full Names
ribonuclease T2A
NCBI Official Symbol
Rnaset2a
NCBI Official Synonym Symbols
C79750; Rnaset2; RNASE6PL; 0610007O07Rik; 4833423A10Rik; 4930532K22Rik
NCBI Protein Information
ribonuclease T2; ribonuclease 6
UniProt Protein Name
Ribonuclease T2
Protein Family
UniProt Gene Name
Rnaset2
UniProt Synonym Gene Names
Rnase6pl
UniProt Entry Name
RNT2_MOUSE
Uniprot Description
RNASET2: Has ribonuclease activity, with higher activity at acidic pH. May play a role in cellular RNA catabolism. Defects in RNASET2 are the cause of leukoencephalopathy cystic without megalencephaly (LCWM). An infantile- onset syndrome of cerebral leukoencephalopathy. Affected newborns develop microcephaly and neurologic abnormalities including psychomotor impairment, seizures and sensorineural hearing impairment. The brain shows multifocal white matter lesions, anterior temporal lobe subcortical cysts, pericystic abnormal myelination, ventriculomegaly and intracranial calcifications. Belongs to the RNase T2 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; EC 3.1.27.-; Secreted; Ribonuclease; RNA-binding
Cellular Component: extracellular space; endoplasmic reticulum lumen; lysosome
Molecular Function: ribonuclease activity
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Product Notes
The Sheep RNASET2 rnaset2 (Catalog #AAA753784) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA753784 ELISA Kit recognizes Sheep RNASET2. It is sometimes possible for the material contained within the vial of "Ribonuclease T2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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