Typical Testing Data/Standard Curve (for reference only)
Chicken Urocortin ELISA Kit | UCN elisa kit
Chicken Urocortin ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: UCN ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-UCN antibody and an UCN-HRP conjugate. The assay sample and buffer are incubated together with UCN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the UCN concentration since UCN from samples and UCN-HRP conjugate compete for the anti-UCN antibody binding site. Since the number of sites is limited, as more sites are occupied by UCN from the sample, fewer sites are left to bind UCN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The UCN concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
Uniprot Description
UCN: Acts in vitro to stimulate the secretion of adrenocorticotropic hormone (ACTH). Binds with high affinity to CRF receptor types 1, 2-alpha, and 2-beta. Belongs to the sauvagine/corticotropin-releasing factor/urotensin I family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 2p23.3
Cellular Component: dendrite; extracellular region; perikaryon; nerve terminal
Molecular Function: corticotropin-releasing hormone receptor 1 binding; histone deacetylase inhibitor activity; neuropeptide hormone activity; protein binding; corticotropin-releasing hormone receptor 2 binding
Biological Process: regulation of synaptic transmission, glutamatergic; positive regulation of translation; negative regulation of hormone secretion; response to glucocorticoid stimulus; positive regulation of collagen biosynthetic process; drinking behavior; negative regulation of cell size; response to pain; female pregnancy; positive regulation of vasodilation; pancreatic juice secretion; negative regulation of appetite; response to estradiol stimulus; positive regulation of adrenocorticotropic hormone secretion; sensory perception of sound; neuropeptide signaling pathway; positive regulation of cAMP biosynthetic process; negative regulation of blood pressure; inflammatory response; neurite development; associative learning; negative regulation of histone deacetylation; activation of protein kinase A; positive regulation of interleukin-6 production; social behavior; positive regulation of peptidyl-serine phosphorylation; positive regulation of cell growth; positive regulation of vascular permeability; G-protein coupled receptor protein signaling pathway; aerobic respiration; response to oxidative stress; positive regulation of DNA replication; negative regulation of apoptosis
Similar Products
Product Notes
The Chicken UCN ucn (Catalog #AAA753707) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA753707 ELISA Kit recognizes Chicken UCN. It is sometimes possible for the material contained within the vial of "Urocortin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
