Human Interleukin 6 receptor ELISA Kit | IL 6R elisa kit

Human Interleukin 6 receptor ELISA Kit

Cat. Num. AAA751117

• Gene Names: IL6R; IL6Q; gp80; CD126; IL6RA; IL6RQ; IL-6RA; IL-6R-1
• Reactivity: Human
• Synonyms: Interleukin 6 receptor; Human Interleukin 6 receptor ELISA Kit; IL 6R elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 50-1000 pg/mL
• Sensitivity: 1.0 pg/mL
• Intended Uses: This IL-6R ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human IL-6R. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL 6R elisa kit

Principle of the assay: IL-6R ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IL-6R antibody and an IL-6R-HRP conjugate. The assay sample and buffer are incubated together with IL-6R-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-6R concentration since IL-6R from samples and IL-6R-HRP conjugate compete for the anti-IL-6R antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-6R from the sample, fewer sites are left to bind IL-6R-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-6R concentration in each sample is interpolated from this standard curve.

Product Categories/Family for IL 6R elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 332309226
• NCBI GeneID: 3570
• NCBI Accession #: NP_001193795.1
• NCBI GenBank Nucleotide #: NM_001206866.1
• Molecular Weight: 40,237 Da
• NCBI Official Full Name: interleukin-6 receptor subunit alpha isoform 3
• NCBI Official Synonym Full Names: interleukin 6 receptor
• NCBI Official Symbol: IL6R
• NCBI Official Synonym Symbols: IL6Q; gp80; CD126; IL6RA; IL6RQ; IL-6RA; IL-6R-1
• NCBI Protein Information: interleukin-6 receptor subunit alpha; IL-6R 1; CD126 antigen; membrane glycoprotein 80; IL-6 receptor subunit alpha
• UniProt Protein Name: Interleukin-6 receptor subunit alpha
• UniProt Gene Name: IL6R
• UniProt Synonym Gene Names: IL-6 receptor subunit alpha; IL-6R subunit alpha; IL-6R-alpha; IL-6RA; gp80
• UniProt Entry Name: IL6RA_HUMAN

NCBI Description

This gene encodes a subunit of the interleukin 6 (IL6) receptor complex. Interleukin 6 is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in the immune response. The IL6 receptor is a protein complex consisting of this protein and interleukin 6 signal transducer (IL6ST/GP130/IL6-beta), a receptor subunit also shared by many other cytokines. Dysregulated production of IL6 and this receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. Alternatively spliced transcript variants encoding distinct isoforms have been reported. A pseudogene of this gene is found on chromosome 9.[provided by RefSeq, May 2011]

Uniprot Description

IL6R: Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis. Belongs to the type I cytokine receptor family. Type 3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: extracellular space; basolateral plasma membrane; apical plasma membrane; extracellular region; plasma membrane; interleukin-6 receptor complex

Molecular Function: protein binding; protein homodimerization activity; enzyme binding; interleukin-6 binding; interleukin-6 receptor binding; ciliary neurotrophic factor receptor activity; interleukin-6 receptor activity

Biological Process: cytokine and chemokine mediated signaling pathway; negative regulation of collagen biosynthetic process; positive regulation of smooth muscle cell proliferation; neutrophil mediated immunity; positive regulation of interleukin-6 production; positive regulation of leukocyte chemotaxis; defense response to Gram-negative bacterium; endocrine pancreas development; positive regulation of chemokine production; activation of NF-kappaB transcription factor; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of osteoblast differentiation; monocyte chemotaxis; defense response to Gram-positive bacterium; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of MAPKKK cascade; response to cytokine stimulus; positive regulation of cell proliferation; negative regulation of interleukin-8 production; acute-phase response; hepatic immune response

Disease: Interleukin 6, Serum Level Of, Quantitative Trait Locus; Soluble Interleukin-6 Receptor, Serum Level Of, Quantitative Trait Locus