Porcine Retinoic Acid Early Transcript 1G ELISA Kit | RAET1G elisa kit

Porcine Retinoic Acid Early Transcript 1G ELISA Kit

Cat. Num. AAA747139

• Gene Names: RAET1G; ULBP5
• Reactivity: Porcine
• Synonyms: Retinoic Acid Early Transcript 1G; Porcine Retinoic Acid Early Transcript 1G ELISA Kit; RAET1G elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This RAET1G ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine RAET1G. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RAET1G elisa kit

Principle of the assay: RAET1G ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RAET1G antibody and an RAET1G-HRP conjugate. The assay sample and buffer are incubated together with RAET1G-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RAET1G concentration since RAET1G from samples and RAET1G-HRP conjugate compete for the anti-RAET1G antibody binding site. Since the number of sites is limited, as more sites are occupied by RAET1G from the sample, fewer sites are left to bind RAET1G-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RAET1G concentration in each sample is interpolated from this standard curve.

Product Categories/Family for RAET1G elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 130484306
• NCBI GeneID: 353091
• NCBI Accession #: NP_001001788.2
• NCBI GenBank Nucleotide #: NM_001001788.2
• UniProt Accession #: Q6H3X3; Q6H3X2
• Molecular Weight: 37,106 Da
• NCBI Official Full Name: retinoic acid early transcript 1G protein
• NCBI Official Synonym Full Names: retinoic acid early transcript 1G
• NCBI Official Symbol: RAET1G
• NCBI Official Synonym Symbols: ULBP5
• NCBI Protein Information: retinoic acid early transcript 1G protein; retinoic acid early transcript 1G pseudogene
• UniProt Protein Name: Retinoic acid early transcript 1G protein
• Protein Family: Retinoic acid early transcript 1G protein
• UniProt Gene Name: RAET1G
• UniProt Entry Name: RET1G_HUMAN

NCBI Description

Members of the RAET1 family, such as RAET1G, are major histocompatibility complex (MHC) class I-related genes located within a 180-kb cluster on chromosome 6q24.2-q25.3. RAET1 proteins contain MHC class I-like alpha-1 and alpha-2 domains. RAET1E (MIM 609243) and RAET1G differ from the other RAET1 proteins (e.g., RAET1I, or ULBP1; MIM 605697) in that they have type I membrane-spanning sequences at their C termini rather than glycosylphosphatidylinositol anchor sequences. (Radosavljevic et al., 2002 [PubMed 11827464]).[supplied by OMIM, Mar 2008]

Uniprot Description

RAET1G: Acts as a ligand for the NKG2D receptor and mediates NK cell cytotoxicity via the receptor. Belongs to the MHC class I family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6q24.1-q25.1

Cellular Component: endoplasmic reticulum; extracellular region; plasma membrane; integral to membrane

Molecular Function: peptide antigen binding; natural killer cell lectin-like receptor binding

Biological Process: antigen processing and presentation of peptide antigen via MHC class I; natural killer cell mediated cytotoxicity