Typical Testing Data/Standard Curve (for reference only)
Bovine Thymidine Kinase ELISA Kit | TK elisa kit
Bovine Thymidine Kinase ELISA Kit
Gene Names
Tk1; Tk-1; Tk1a; Tk1b; D530002A18Rik
Reactivity
Bovine
Synonyms
Thymidine Kinase; Bovine Thymidine Kinase ELISA Kit; TK elisa kit
Reactivity
Bovine
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Intended Uses
This TK ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine TK. This ELISA kit for research use only, not for therapeutic applications
!Intended Uses
This TK ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine TK. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for TK elisa kit
Principle of the Assay: TK ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TK antibody and an TK-HRP conjugate. The assay sample and buffer are incubated together with TK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TK concentration since TK from samples and TK-HRP conjugate compete for the anti-TK antibody binding site. Since the number of sites is limited, as more sites are occupied by TK from the sample, fewer sites are left to bind TK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TK concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
25,776 Da
NCBI Official Full Name
thymidine kinase
NCBI Official Synonym Full Names
thymidine kinase 1
NCBI Official Symbol
Tk1
NCBI Official Synonym Symbols
Tk-1; Tk1a; Tk1b; D530002A18Rik
NCBI Protein Information
thymidine kinase, cytosolic
UniProt Protein Name
Thymidine kinase, cytosolic
Protein Family
UniProt Gene Name
Tk1
UniProt Synonym Gene Names
Tk-1
UniProt Entry Name
KITH_MOUSE
Uniprot Description
TK: cytosolic thymidine kinase. Phosphorylated during mitosis. Its enzymatic activity is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.
Protein type: Xenobiotic Metabolism - drug metabolism - other enzymes; EC 2.7.1.21; Kinase, other; Nucleotide Metabolism - pyrimidine
Cellular Component: cytoplasm
Molecular Function: transferase activity; identical protein binding; nucleoside kinase activity; zinc ion binding; metal ion binding; nucleotide binding; thymidine kinase activity; kinase activity; ATP binding
Biological Process: nucleotide biosynthetic process; thymidine metabolic process; phosphorylation; deoxyribonucleoside monophosphate biosynthetic process; protein homotetramerization
Research Articles on TK
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Product Notes
The Bovine TK tk1 (Catalog #AAA746734) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA746734 ELISA Kit recognizes Bovine TK. It is sometimes possible for the material contained within the vial of "Thymidine Kinase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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