Chicken Calcium/calmodulin-dependent protein kinase-II ELISA Kit | CAMK2 elisa kit

Chicken Calcium/calmodulin-dependent protein kinase-II ELISA Kit

Cat. Num. AAA746402

• Gene Names: CAMK2B; CAM2; CAMK2; CAMKB
• Reactivity: Chicken
• Synonyms: Calcium/calmodulin-dependent protein kinase-II; Chicken Calcium/calmodulin-dependent protein kinase-II ELISA Kit; CAMK2 elisa kit

• Reactivity: Chicken

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Intended Uses: This CAMK2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken CAMK2. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This CAMK2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken CAMK2. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CAMK2 elisa kit

Principle of the Assay: CAMK2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CAMK2 antibody and an CAMK2-HRP conjugate. The assay sample and buffer are incubated together with CAMK2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CAMK2 concentration since CAMK2 from samples and CAMK2-HRP conjugate compete for the anti-CAMK2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CAMK2 from the sample, fewer sites are left to bind CAMK2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CAMK2 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CAMK2 elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 26051218
• NCBI GeneID: 816
• NCBI Accession #: NP_742081.1
• NCBI GenBank Nucleotide #: NM_172084.2
• Molecular Weight: 57,938 Da
• NCBI Official Full Name: calcium/calmodulin-dependent protein kinase type II subunit beta isoform 8
• NCBI Official Synonym Full Names: calcium/calmodulin-dependent protein kinase II beta
• NCBI Official Symbol: CAMK2B
• NCBI Official Synonym Symbols: CAM2; CAMK2; CAMKB
• NCBI Protein Information: calcium/calmodulin-dependent protein kinase type II subunit beta; caMK-II subunit beta; CaM-kinase II beta chain; CaM kinase II beta subunit; proline rich calmodulin-dependent protein kinase
• UniProt Protein Name: Calcium/calmodulin-dependent protein kinase type II subunit beta
• UniProt Gene Name: CAMK2B
• UniProt Synonym Gene Names: CAM2; CAMK2; CAMKB; CaM kinase II subunit beta; CaMK-II subunit beta
• UniProt Entry Name: KCC2B_HUMAN

NCBI Description

The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a beta chain. It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Uniprot Description

CAMK2B: a widely expressed protein kinase of the calcium/calmodulin-dependent protein kinase (CAMK) group. Functions autonomously after Ca2+/calmodulin-binding and autophosphorylation. Involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca2+ transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. Participates in the modulation of skeletal muscle function in response to exercise. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca2+ transport and in fast-twitch muscle participates in the control of Ca2+ release from the SR through phosphorylation of triadin, a ryanodine receptor-coupling factor, and phospholamban, an endogenous inhibitor of SERCA2. The holoenzyme is composed of 4 different chains: alpha (CAMK2A), beta (CAMK2B), gamma (CAMK2G), and delta (CAMK2D). The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 12 subunits with two hexameric rings stacked one on top of the other. Interacts with SYNGAP1 and CAMK2N2. Interacts with MPDZ. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain. Expressed in skeletal muscle and induced during exercise. Eight isoforms of the human protein are produced by alternative splicing

Protein type: Kinase, protein; Protein kinase, CAMK; EC 2.7.11.17; Protein kinase, Ser/Thr (non-receptor); CAMK group; CAMK2 family

Chromosomal Location of Human Ortholog: 7p14.3-p14.1

Cellular Component: nucleoplasm; sarcoplasmic reticulum membrane; plasma membrane; microtubule organizing center; spindle midzone; cytosol

Molecular Function: calmodulin binding; protein homodimerization activity; calmodulin-dependent protein kinase activity; actin binding; ATP binding

Biological Process: regulation of long-term neuronal synaptic plasticity; regulation of skeletal muscle adaptation; cytokine and chemokine mediated signaling pathway; protein amino acid autophosphorylation; regulation of calcium ion transport; signal transduction; protein amino acid phosphorylation; regulation of synaptic transmission, cholinergic; synaptic transmission; inhibitory G-protein coupled receptor phosphorylation; peptidyl-serine phosphorylation; response to cadmium ion; calcium ion transport; regulation of synapse structural plasticity; neuromuscular process controlling balance; G1/S transition of mitotic cell cycle