Sheep Urocortin ELISA Kit | UCN elisa kit

Sheep Urocortin ELISA Kit

Cat. Num. AAA745753

• Reactivity: Sheep
• Synonyms: Urocortin; Sheep Urocortin ELISA Kit; UCN elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 5.0-100ng/mL
• Sensitivity: 1.0ng/mL
• Intended Uses: This UCN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep UCN. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for UCN elisa kit

Principle of the Assay: UCN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-UCN antibody and an UCN-HRP conjugate. The assay sample and buffer are incubated together with UCN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the UCN concentration since UCN from samples and UCN-HRP conjugate compete for the anti-UCN antibody binding site. Since the number of sites is limited, as more sites are occupied by UCN from the sample, fewer sites are left to bind UCN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The UCN concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 3252877
• Molecular Weight: 13,458 Da
• NCBI Official Full Name: urocortin
• UniProt Protein Name: Urocortin
• Protein Family: Urocortin
• UniProt Gene Name: UCN
• UniProt Entry Name: UCN1_HUMAN

Uniprot Description

UCN: Acts in vitro to stimulate the secretion of adrenocorticotropic hormone (ACTH). Binds with high affinity to CRF receptor types 1, 2-alpha, and 2-beta. Belongs to the sauvagine/corticotropin-releasing factor/urotensin I family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2p23.3

Cellular Component: dendrite; extracellular region; perikaryon; nerve terminal

Molecular Function: corticotropin-releasing hormone receptor 1 binding; histone deacetylase inhibitor activity; neuropeptide hormone activity; protein binding; corticotropin-releasing hormone receptor 2 binding

Biological Process: regulation of synaptic transmission, glutamatergic; positive regulation of translation; negative regulation of hormone secretion; drinking behavior; positive regulation of collagen biosynthetic process; response to glucocorticoid stimulus; negative regulation of cell size; response to pain; female pregnancy; pancreatic juice secretion; positive regulation of vasodilation; response to estradiol stimulus; negative regulation of appetite; positive regulation of adrenocorticotropic hormone secretion; sensory perception of sound; neuropeptide signaling pathway; positive regulation of cAMP biosynthetic process; negative regulation of blood pressure; inflammatory response; neurite development; associative learning; negative regulation of histone deacetylation; activation of protein kinase A; positive regulation of interleukin-6 production; social behavior; positive regulation of peptidyl-serine phosphorylation; positive regulation of cell growth; positive regulation of vascular permeability; G-protein coupled receptor protein signaling pathway; aerobic respiration; response to oxidative stress; positive regulation of DNA replication; negative regulation of apoptosis