Canine Heterogeneous nuclear ribonucleoprotein A2 ELISA Kit | hnRNP A2 elisa kit

Canine Heterogeneous nuclear ribonucleoprotein A2 ELISA Kit

Cat. Num. AAA745662

• Gene Names: HNRNPA2B1; RNPA2; HNRPA2; HNRPB1; SNRPB1; HNRNPA2; HNRNPB1; IBMPFD2; HNRPA2B1
• Reactivity: Canine
• Synonyms: Heterogeneous nuclear ribonucleoprotein A2; Canine Heterogeneous nuclear ribonucleoprotein A2 ELISA Kit; hnRNP A2 elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.
• Intended Uses: This hnRNP A2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine hnRNP A2. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for hnRNP A2 elisa kit

Principle of the assay: hnRNP A2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-hnRNP A2 antibody and an hnRNP A2-HRP conjugate. The assay sample and buffer are incubated together with hnRNP A2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the hnRNP A2 concentration since hnRNP A2 from samples and hnRNP A2-HRP conjugate compete for the anti-hnRNP A2 antibody binding site. Since the number of sites is limited, as more sites are occupied by hnRNP A2 from the sample, fewer sites are left to bind hnRNP A2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The hnRNP A2 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for hnRNP A2 elisa kit

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 565644
• NCBI GeneID: 3181
• Molecular Weight: 36,006 Da
• NCBI Official Full Name: hnRNP A2 protein
• NCBI Official Synonym Full Names: heterogeneous nuclear ribonucleoprotein A2/B1
• NCBI Official Symbol: HNRNPA2B1
• NCBI Official Synonym Symbols: RNPA2; HNRPA2; HNRPB1; SNRPB1; HNRNPA2; HNRNPB1; IBMPFD2; HNRPA2B1
• NCBI Protein Information: heterogeneous nuclear ribonucleoproteins A2/B1; hnRNP A2 / hnRNP B1; nuclear ribonucleoprotein particle A2 protein
• UniProt Protein Name: Heterogeneous nuclear ribonucleoproteins A2/B1
• Protein Family: Heterogeneous nuclear ribonucleoprotein
• UniProt Gene Name: HNRNPA2B1
• UniProt Synonym Gene Names: HNRPA2B1; hnRNP A2/B1
• UniProt Entry Name: ROA2_HUMAN

NCBI Description

This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. This gene has been described to generate two alternatively spliced transcript variants which encode different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

hnRNP A2/B1: Involved with pre-mRNA processing. Forms complexes (ribonucleosomes) with at least 20 other different hnRNP and heterogeneous nuclear RNA in the nucleus. Identified in the spliceosome C complex. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with IGF2BP1. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; RNA splicing; Spliceosome

Chromosomal Location of Human Ortholog: 7p15

Cellular Component: nucleoplasm; spliceosome; membrane; cytoplasm; nucleus; ribonucleoprotein complex

Molecular Function: protein binding; single-stranded telomeric DNA binding; RNA binding; nucleotide binding

Biological Process: nuclear mRNA splicing, via spliceosome; negative regulation of nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression; negative regulation of transcription from RNA polymerase II promoter; mRNA processing; RNA transport

Disease: Inclusion Body Myopathy With Early-onset Paget Disease With Or Without Frontotemporal Dementia 2