Wingless-type MMTV integration site family, member 4 ELISA Kit | WTN-4 elisa kit

Rat Wingless-type MMTV integration site family, member 4 ELISA Kit

Cat. Num. AAA744493

• Gene Names: WNT4; WNT-4; SERKAL
• Reactivity: Rat
• Synonyms: Wingless-type MMTV integration site family; member 4; Rat Wingless-type MMTV integration site family; member 4 ELISA Kit; WTN-4 elisa kit

• Reactivity: Rat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/ml
• Intended Uses: This WNT4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of RatWNT4. This ELISA kit for research use only, not for therapeutic applications
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for WTN-4 elisa kit

Principle of the Assay: WNT4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-WNT4 antibody and an WNT4-HRP conjugate. The assay sample and buffer are incubated together with WNT4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the WNT4 concentration since WNT4 from samples and WNT4-HRP conjugate compete for the anti-WNT4 antibody binding site. Since the number of sites is limited, as more sites are occupied by WNT4 from the sample, fewer sites are left to bind WNT4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The WNT4 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for WTN-4 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 34785937
• NCBI GeneID: 54361
• Molecular Weight: 32,954 Da
• NCBI Official Full Name: Wingless-type MMTV integration site family, member 4
• NCBI Official Synonym Full Names: wingless-type MMTV integration site family, member 4
• NCBI Official Symbol: WNT4
• NCBI Official Synonym Symbols: WNT-4; SERKAL
• NCBI Protein Information: protein Wnt-4
• UniProt Protein Name: Protein Wnt-4
• UniProt Gene Name: WNT4
• UniProt Synonym Gene Names: UNQ426/PRO864
• UniProt Entry Name: WNT4_HUMAN

NCBI Description

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family, and is the first signaling molecule shown to influence the sex-determination cascade. It encodes a protein which shows 98% amino acid identity to the Wnt4 protein of mouse and rat. This gene and a nuclear receptor known to antagonize the testis-determining factor play a concerted role in both the control of female development and the prevention of testes formation. This gene and another two family members, WNT2 and WNT7B, may be associated with abnormal proliferation in breast tissue. Mutations in this gene can result in Rokitansky-Kuster-Hauser syndrome and in SERKAL syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

WNT4: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Overexpression may be associated with abnormal proliferation in human breast tissue. Defects in WNT4 are a cause of Rokitansky-Kuster-Hauser syndrome (RKH syndrome); also called Mayer- Rokitansky-Kuster-Hauser syndrome (MRKH syndrome or MRKH anomaly). RKH syndrome is characterized by utero-vaginal atresia in otherwise phenotypically normal female with a normal 46,XX karyotype. Anomalies of the genital tract range from upper vaginal atresia to total Muellerian agenesis with urinary tract abnormalities. It has an incidence of approximately 1 in 5'000 newborn girls. Defects in WNT4 are the cause of female sex reversal with dysgenesis of kidneys, adrenals, and lungs (SERKAL); also known as SERKAL syndrome. Defects in WNT4 are the cause of Muellerian aplasia (MULLAPL). Belongs to the Wnt family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p36.23-p35.1

Cellular Component: proteinaceous extracellular matrix; extracellular space; cell surface; Golgi lumen; endoplasmic reticulum lumen; cytoplasm; plasma membrane; extracellular region

Molecular Function: frizzled binding; transcription corepressor activity; receptor agonist activity

Biological Process: negative regulation of fibroblast growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; positive regulation of collagen biosynthetic process; thyroid stimulating hormone secreting cell differentiation; androgen biosynthetic process; Wnt receptor signaling pathway through beta-catenin; somatotropin secreting cell differentiation; neuron differentiation; regulation of cell-cell adhesion; positive regulation of focal adhesion formation; positive regulation of aldosterone biosynthetic process; positive regulation of stress fiber formation; embryonic epithelial tube formation; kidney development; negative regulation of cell migration; female gonad development; cell fate commitment; positive regulation of meiosis; adrenal gland development; male gonad development; immature T cell proliferation in the thymus; liver development; cellular response to starvation; positive regulation of bone mineralization; positive regulation of osteoblast differentiation; negative regulation of cell differentiation; protein palmitoylation; ureteric bud branching; epithelial to mesenchymal transition; female sex determination; smooth muscle cell differentiation; negative regulation of transcription, DNA-dependent; oocyte development

Disease: Mullerian Aplasia And Hyperandrogenism; 46,xx Sex Reversal With Dysgenesis Of Kidneys, Adrenals, And Lungs