Monkey G Protein Beta 1 ELISA Kit | GNb1 elisa kit

Monkey G Protein Beta 1 ELISA Kit

Cat. Num. AAA743381

• Reactivity: Monkey
• Synonyms: G Protein Beta 1; Monkey G Protein Beta 1 ELISA Kit; GNb1 elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This GNb1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey GNb1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GNb1 elisa kit

Principle of the Assay: GNb1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GNb1 antibody and an GNb1-HRP conjugate. The assay sample and buffer are incubated together with GNb1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GNb1 concentration since GNb1 from samples and GNb1-HRP conjugate compete for the anti-GNb1 antibody binding site. Since the number of sites is limited, as more sites are occupied by GNb1 from the sample, fewer sites are left to bind GNb1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GNb1 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 48145685
• NCBI GeneID: 2782
• UniProt Accession #: P62873; P04697; P04901; Q1RMY8; B1AJZ7
• Molecular Weight: 36,298 Da
• NCBI Official Full Name: GNB1
• NCBI Official Synonym Full Names: guanine nucleotide binding protein (G protein), beta polypeptide 1
• NCBI Official Symbol: GNB1
• NCBI Protein Information: guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1; guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1; transducin beta chain 1; G protein, beta-1 subunit; beta subunit, signal-transducing proteins GS/GI
• UniProt Protein Name: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
• UniProt Gene Name: GNB1
• UniProt Entry Name: GBB1_HUMAN

NCBI Description

Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Uniprot Description

G-beta 1: Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein- effector interaction. Belongs to the WD repeat G protein beta family.

Protein type: G protein, heterotrimeric; G protein; G protein, heterotrimeric beta

Chromosomal Location of Human Ortholog: 1p36.33

Cellular Component: photoreceptor inner segment; membrane; lysosomal membrane; dendrite; plasma membrane; heterotrimeric G-protein complex; intracellular; vesicle

Molecular Function: GTPase activity; GTPase binding; protein binding; signal transducer activity; spectrin binding; protein complex binding; type 1 angiotensin receptor binding

Biological Process: phototransduction, visible light; platelet activation; acetylcholine receptor signaling, muscarinic pathway; sensory perception of taste; signal transduction; rhodopsin mediated signaling; cell proliferation; synaptic transmission; elevation of cytosolic calcium ion concentration; regulation of rhodopsin mediated signaling; retina development in camera-type eye; Ras protein signal transduction; energy reserve metabolic process; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); blood coagulation; dopamine receptor, adenylate cyclase activating pathway