Sheep Interleukin 9 ELISA Kit | IL-9 elisa kit

Sheep Interleukin 9 ELISA Kit

Cat. Num. AAA743049

• Gene Names: IL9; P40; HP40; IL-9
• Reactivity: Sheep
• Synonyms: Interleukin 9; Sheep Interleukin 9 ELISA Kit; IL-9 elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.
• Intended Uses: This IL-9 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep IL-9. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-9 elisa kit

Principle of the Assay: IL-9 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IL-9 antibody and an IL-9-HRP conjugate. The assay sample and buffer are incubated together with IL-9-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-9 concentration since IL-9 from samples and IL-9-HRP conjugate compete for the anti-IL-9 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-9 from the sample, fewer sites are left to bind IL-9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-9 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for IL-9 elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 10834980
• NCBI GeneID: 3578
• NCBI Accession #: NP_000581.1
• NCBI GenBank Nucleotide #: NM_000590.1
• Molecular Weight: 15,909 Da
• NCBI Official Full Name: interleukin-9
• NCBI Official Synonym Full Names: interleukin 9
• NCBI Official Symbol: IL9
• NCBI Official Synonym Symbols: P40; HP40; IL-9
• NCBI Protein Information: interleukin-9; cytokine P40; p40 cytokine; T-cell growth factor p40; p40 T-cell and mast cell growth factor; homolog of mouse T cell and mast cell growth factor 40
• UniProt Protein Name: Interleukin-9
• UniProt Gene Name: IL9
• UniProt Synonym Gene Names: IL-9
• UniProt Entry Name: IL9_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that acts as a regulator of a variety of hematopoietic cells. This cytokine stimulates cell proliferation and prevents apoptosis. It functions through the interleukin 9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. The gene encoding this cytokine has been identified as a candidate gene for asthma. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyperresponsiveness. [provided by RefSeq, Jul 2008]

Uniprot Description

IL9: Supports IL-2 independent and IL-4 independent growth of helper T-cells. Belongs to the IL-7/IL-9 family.

Protein type: Secreted; Secreted, signal peptide; Cytokine

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular space

Molecular Function: growth factor activity; interleukin-9 receptor binding; cytokine activity

Biological Process: positive regulation of cell proliferation; immune response; inflammatory response; positive regulation of cell growth; positive regulation of interleukin-5 biosynthetic process