Canine Triggering Receptor Expresses on Myeloid Cells-1 ELISA Kit | TREM-1 elisa kit

Canine Triggering Receptor Expresses on Myeloid Cells-1 ELISA Kit

Cat. Num. AAA742914

• Gene Names: TREM1; CD354; TREM-1
• Reactivity: Canine
• Synonyms: Triggering Receptor Expresses on Myeloid Cells-1; Canine Triggering Receptor Expresses on Myeloid Cells-1 ELISA Kit; TREM-1 elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 50-1000pg/mL
• Sensitivity: 1.0pg/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TREM-1 elisa kit

Intended Uses: This TREM-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine TREM-1. This ELISA kit for research use only!

Principle of the Assay: TREM-1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for TREM-1. Standards or samples are then added to the microtiter plate wells and TREM-1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of TREM-1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for TREM-1 are added to each well to "sandwich" the TREM-1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TREM-1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TREM-1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for TREM-1 elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 8924262
• NCBI GeneID: 54210
• NCBI Accession #: NP_061113.1
• NCBI GenBank Nucleotide #: NM_018643.3
• Molecular Weight: 25,574 Da
• NCBI Official Full Name: triggering receptor expressed on myeloid cells 1 isoform 1
• NCBI Official Synonym Full Names: triggering receptor expressed on myeloid cells 1
• NCBI Official Symbol: TREM1
• NCBI Official Synonym Symbols: CD354; TREM-1
• NCBI Protein Information: triggering receptor expressed on myeloid cells 1; triggering-receptor TREM1; triggering receptor expressed on monocytes 1
• UniProt Protein Name: Triggering receptor expressed on myeloid cells 1
• UniProt Gene Name: TREM1
• UniProt Synonym Gene Names: TREM-1
• UniProt Entry Name: TREM1_HUMAN

NCBI Description

This gene encodes a receptor belonging to the Ig superfamily that is expressed on myeloid cells. This protein amplifies neutrophil and monocyte-mediated inflammatory responses triggered by bacterial and fungal infections by stimulating release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Jun 2011]

Uniprot Description

TREM1: Stimulates neutrophil and monocyte-mediated inflammatory responses. Triggers release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Amplifier of inflammatory responses that are triggered by bacterial and fungal infections and is a crucial mediator of septic shock. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: extracellular region; plasma membrane; integral to membrane

Molecular Function: receptor activity

Biological Process: neutrophil chemotaxis; innate immune response; cytokine secretion during immune response; blood coagulation; leukocyte migration; chemokine metabolic process; humoral immune response