Canine Prostaglandin E Synthase, Microsomal ELISA Kit | PTGES elisa kit

Canine Prostaglandin E Synthase, Microsomal ELISA Kit

Cat. Num. AAA741867

• Gene Names: PTGES; PGES; MPGES; PIG12; PP102; PP1294; MGST-IV; MGST1L1; TP53I12; mPGES-1; MGST1-L1
• Reactivity: Canine
• Synonyms: Prostaglandin E Synthase; Microsomal; Canine Prostaglandin E Synthase; Microsomal ELISA Kit; PTGES elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PTGES elisa kit

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

INTENDED USE This PTGES ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine PTGES. This ELISA kit for research use only!

PRINCIPLE OF THE ASSAY PTGES ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PTGES antibody and an PTGES-HRP conjugate. The assay sample and buffer are incubated together with PTGES-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PTGES concentration since PTGES from samples and PTGES-HRP conjugate compete for the anti-PTGES antibody binding site. Since the number of sites is limited, as more sites are occupied by PTGES from the sample, fewer sites are left to bind PTGES-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PTGES concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4758910
• NCBI GeneID: 9536
• NCBI Accession #: NP_004869.1
• NCBI GenBank Nucleotide #: NM_004878.4
• Molecular Weight: 17,102 Da
• NCBI Official Full Name: prostaglandin E synthase
• NCBI Official Synonym Full Names: prostaglandin E synthase
• NCBI Official Symbol: PTGES
• NCBI Official Synonym Symbols: PGES; MPGES; PIG12; PP102; PP1294; MGST-IV; MGST1L1; TP53I12; mPGES-1; MGST1-L1
• NCBI Protein Information: prostaglandin E synthase; MGST1-like 1; p53-induced gene 12 protein; p53-induced apoptosis protein 12; glutathione S-transferase 1-like 1; microsomal prostaglandin E synthase 1; microsomal prostaglandin E synthase-1; tumor protein p53 inducible protein 12; microsomal glutathione S-transferase 1-like 1
• UniProt Protein Name: Prostaglandin E synthase
• Protein Family: Prostaglandin E synthase
• UniProt Gene Name: PTGES
• UniProt Synonym Gene Names: MGST1L1; MPGES1; PGES; PIG12; MGST1-L1; MPGES-1
• UniProt Entry Name: PTGES_HUMAN

NCBI Description

The protein encoded by this gene is a glutathione-dependent prostaglandin E synthase. The expression of this gene has been shown to be induced by proinflammatory cytokine interleukin 1 beta (IL1B). Its expression can also be induced by tumor suppressor protein TP53, and may be involved in TP53 induced apoptosis. Knockout studies in mice suggest that this gene may contribute to the pathogenesis of collagen-induced arthritis and mediate acute pain during inflammatory responses. [provided by RefSeq, Jul 2008]

Uniprot Description

PTGES: Catalyzes the oxidoreduction of prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2). Belongs to the MAPEG family.

Protein type: Membrane protein, integral; Isomerase; EC 5.3.99.3; Membrane protein, multi-pass; Nuclear envelope; Lipid Metabolism - arachidonic acid

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: endoplasmic reticulum membrane; membrane; perinuclear region of cytoplasm; nuclear envelope lumen; integral to membrane

Molecular Function: glutathione binding; prostaglandin-E synthase activity

Biological Process: chronic inflammatory response; response to retinoic acid; cyclooxygenase pathway; response to lipopolysaccharide; signal transduction; prostaglandin biosynthetic process; response to organic cyclic substance; prostaglandin metabolic process; negative regulation of cell proliferation; response to cytokine stimulus; arachidonic acid metabolic process; response to calcium ion; acute inflammatory response