Monkey Neuraminidase ELISA Kit | NEU elisa kit

Monkey Neuraminidase ELISA Kit

Cat. Num. AAA741318

• Reactivity: Monkey
• Synonyms: Neuraminidase; Monkey Neuraminidase ELISA Kit; NEU elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 50-1000pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This NA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey NA. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NEU elisa kit

Principle of the Assay: NA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NA antibody and an NA-HRP conjugate. The assay sample and buffer are incubated together with NA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NA concentration since NA from samples and NA-HRP conjugate compete for the anti-NA antibody binding site. Since the number of sites is limited, as more sites are occupied by NA from the sample, fewer sites are left to bind NA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NA concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 8250669
• Molecular Weight: 51,888 Da
• NCBI Official Full Name: neuraminidase
• UniProt Protein Name: Neuraminidase
• Protein Family: Neuraminidase
• UniProt Gene Name: NA
• UniProt Entry Name: NRAM_I57A1

Uniprot Description

Catalyzes the removal of terminal sialic acid residues from viral and cellular glycoconjugates. Cleaves off the terminal sialic acids on the glycosylated HA during virus budding to facilitate virus release. Additionally helps virus spread through the circulation by further removing sialic acids from the cell surface. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell. Otherwise, infection would be limited to one round of replication. Described as a receptor-destroying enzyme because it cleaves a terminal sialic acid from the cellular receptors. May facilitate viral invasion of the upper airways by cleaving the sialic acid moities on the mucin of the airway epithelial cells. Likely to plays a role in the budding process through its association with lipid rafts during intracellular transport. May additionally display a raft-association independent effect on budding. Plays a role in the determination of host range restriction on replication and virulence. Sialidase activity in late endosome/lysosome traffic seems to enhance virus replication.