Sheep Monocyte Chemotactic Protein 3 ELISA Kit | MCP-3 elisa kit

Sheep Monocyte Chemotactic Protein 3 ELISA Kit

Cat. Num. AAA740935

• Reactivity: Sheep
• Synonyms: Monocyte Chemotactic Protein 3; Sheep Monocyte Chemotactic Protein 3 ELISA Kit; MCP-3 elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This MCP-3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep MCP-3. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MCP-3 elisa kit

Principle of the Assay: MCP-3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MCP-3 antibody and an MCP-3-HRP conjugate. The assay sample and buffer are incubated together with MCP-3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MCP-3 concentration since MCP-3 from samples and MCP-3-HRP conjugate compete for the anti-MCP-3 antibody binding site. Since the number of sites is limited, as more sites are occupied by MCP-3 from the sample, fewer sites are left to bind MCP-3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MCP-3 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for MCP-3 elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 313708
• UniProt Accession #: P80098; Q569J6
• Molecular Weight: 11,200 Da
• NCBI Official Full Name: monocyte chemotactic protein-3
• UniProt Protein Name: C-C motif chemokine 7
• UniProt Gene Name: CCL7
• UniProt Synonym Gene Names: MCP3; SCYA6; SCYA7; MCP-3
• UniProt Entry Name: CCL7_HUMAN

Uniprot Description

CCL7: Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q11.2-q12

Cellular Component: extracellular space; extracellular region

Molecular Function: heparin binding; CCR1 chemokine receptor binding; CCR2 chemokine receptor binding; chemokine activity

Biological Process: cellular calcium ion homeostasis; regulation of cell shape; monocyte chemotaxis; cell-cell signaling; eosinophil chemotaxis; response to gamma radiation; immune response; cytoskeleton organization and biogenesis; chemotaxis; signal transduction; inflammatory response; positive regulation of cell migration