Canine Teratocarcinoma Derived Growth Factor 1 ELISA Kit | TDGF1 elisa kit

Canine Teratocarcinoma Derived Growth Factor 1 ELISA Kit

Cat. Num. AAA740843

• Gene Names: TDGF1; CR; CRGF; CRIPTO
• Reactivity: Canine
• Synonyms: Teratocarcinoma Derived Growth Factor 1; Canine Teratocarcinoma Derived Growth Factor 1 ELISA Kit; TDGF1 elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This TDGF1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine TDGF1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TDGF1 elisa kit

Principle of the Assay: TDGF1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for TDGF1. Standards or samples are then added to the microtiter plate wells and TDGF1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of TDGF1 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for TDGF1 are added to each well to "sandwich" the TDGF1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TDGF1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TDGF1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for TDGF1 elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 292494883
• NCBI GeneID: 6997
• NCBI Accession #: NP_001167607.1
• NCBI GenBank Nucleotide #: NM_001174136.1
• Molecular Weight: 21,169 Da
• NCBI Official Full Name: teratocarcinoma-derived growth factor 1 isoform 2
• NCBI Official Synonym Full Names: teratocarcinoma-derived growth factor 1
• NCBI Official Symbol: TDGF1
• NCBI Official Synonym Symbols: CR; CRGF; CRIPTO
• NCBI Protein Information: teratocarcinoma-derived growth factor 1; cripto-1 growth factor; epidermal growth factor-like cripto protein CR1
• UniProt Protein Name: Teratocarcinoma-derived growth factor 1
• Protein Family: Teratocarcinoma-derived growth factor
• UniProt Gene Name: TDGF1
• UniProt Synonym Gene Names: CRIPTO; CRGF
• UniProt Entry Name: TDGF1_HUMAN

NCBI Description

This gene encodes an epidermal growth factor-related protein that contains a cripto, FRL-1, and cryptic domain. The encoded protein is an extracellular, membrane-bound signaling protein that plays an essential role in embryonic development and tumor growth. Mutations in this gene are associated with forebrain defects. Pseudogenes of this gene are found on chromosomes 2, 3, 6, 8, 19 and X. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]

Uniprot Description

TDGF1: Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm. Interacts with the activin type-1 receptor ACVR1B. Preferentially expressed in gastric and colorectal carcinomas than in their normal counterparts. Expressed in breast and lung.

Protein type: Motility/polarity/chemotaxis; Membrane protein, peripheral; Cell adhesion; Cell cycle regulation; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: extrinsic to plasma membrane; Golgi apparatus; extracellular space; cell surface; perinuclear region of cytoplasm; apical plasma membrane; plasma membrane; nucleus; lipid raft

Molecular Function: protein binding; growth factor activity; receptor binding

Biological Process: activation of MAPK activity; somatic stem cell maintenance; heart development; regulation of signal transduction; gastrulation; Wnt receptor signaling pathway through beta-catenin; cardiac muscle cell differentiation; BMP signaling pathway; positive regulation of fibroblast proliferation; mammary gland development; determination of anterior/posterior axis, embryo; positive regulation of cell proliferation; vasculogenesis; cell differentiation; positive regulation of cell-matrix adhesion; epidermal growth factor receptor signaling pathway; in utero embryonic development; morphogenesis of a branching structure; peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; embryonic development; cell migration during sprouting angiogenesis; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of cell migration; negative regulation of apoptosis