Bovine Fibulin 5 ELISA Kit | FBLN5 elisa kit

Bovine Fibulin 5 ELISA Kit

Cat. Num. AAA739278

• Gene Names: FBLN5; EVEC; UP50; ADCL2; ARMD3; DANCE; ARCL1A; FIBL-5
• Reactivity: Bovine
• Synonyms: Fibulin 5; Bovine Fibulin 5 ELISA Kit; FBLN5 elisa kit

• Reactivity: Bovine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL.
• Intended Uses: This FBLN5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine FBLN5 This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FBLN5 elisa kit

Principle of the Assay: FBLN5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FBLN5 antibody and an FBLN5-HRP conjugate. The assay sample and buffer are incubated together with FBLN5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FBLN5 concentration since FBLN5 from samples and FBLN5-HRP conjugate compete for the anti-FBLN5 antibody binding site. Since the number of sites is limited, as more sites are occupied by FBLN5 from the sample, fewer sites are left to bind FBLN5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FBLN5 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for FBLN5 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 19743803
• NCBI GeneID: 10516
• NCBI Accession #: NP_006320.2
• NCBI GenBank Nucleotide #: NM_006329.3
• Molecular Weight: 50,180 Da
• NCBI Official Full Name: fibulin-5
• NCBI Official Synonym Full Names: fibulin 5
• NCBI Official Symbol: FBLN5
• NCBI Official Synonym Symbols: EVEC; UP50; ADCL2; ARMD3; DANCE; ARCL1A; FIBL-5
• NCBI Protein Information: fibulin-5; urine p50 protein; developmental arteries and neural crest EGF-like protein
• UniProt Protein Name: Fibulin-5
• Protein Family: Fibulin
• UniProt Gene Name: FBLN5
• UniProt Synonym Gene Names: DANCE; UNQ184/PRO210; FIBL-5; Dance; UP50
• UniProt Entry Name: FBLN5_HUMAN

NCBI Description

The protein encoded by this gene is a secreted, extracellular matrix protein containing an Arg-Gly-Asp (RGD) motif and calcium-binding EGF-like domains. It promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. It is prominently expressed in developing arteries but less so in adult vessels. However, its expression is reinduced in balloon-injured vessels and atherosclerotic lesions, notably in intimal vascular smooth muscle cells and endothelial cells. Therefore, the protein encoded by this gene may play a role in vascular development and remodeling. Defects in this gene are a cause of autosomal dominant cutis laxa, autosomal recessive cutis laxa type I (CL type I), and age-related macular degeneration type 3 (ARMD3). [provided by RefSeq, Jul 2008]

Uniprot Description

FBLN5: Promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. Could be a vascular ligand for integrin receptors and may play a role in vascular development and remodeling. Defects in FBLN5 are the cause of cutis laxa, autosomal dominant, type 2 (ADCL2). A connective tissue disorder characterized by loose, hyperextensible skin with decreased resilience and elasticity leading to a premature aged appearance. Face, hands, feet, joints, and torso may be differentially affected. Additional variable clinical features are gastrointestinal diverticula, hernia, and genital prolapse. Rare manifestations are pulmonary artery stenosis, aortic aneurysm, bronchiectasis, and emphysema. Defects in FBLN5 are a cause of cutis laxa, autosomal recessive, type 1A (ARCL1A). A connective tissue disorder characterized by loose, hyperextensible skin with decreased resilience and elasticity leading to a premature aged appearance. Face, hands, feet, joints, and torso may be differentially affected. The clinical spectrum of autosomal recessive cutis laxa is highly heterogeneous with respect to organ involvement and severity. Type I autosomal recessive cutis laxa is a specific, life-threatening disorder with organ involvement, lung atelectasis and emphysema, diverticula of the gastrointestinal and genitourinary systems, and vascular anomalies. Associated cranial anomalies, late closure of the fontanel, joint laxity, hip dislocation, and inguinal hernia have been observed but are uncommon. Defects in FBLN5 are the cause of age-related macular degeneration type 3 (ARMD3). ARMD is a multifactorial disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid (known as drusen) that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane. Belongs to the fibulin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 14q32.1

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; extracellular region

Molecular Function: protein C-terminus binding; integrin binding; protein binding; protein homodimerization activity; calcium ion binding

Biological Process: elastic fiber assembly; secretion; extracellular matrix organization and biogenesis; cell-matrix adhesion; regulation of cell growth

Disease: Cutis Laxa, Autosomal Dominant 2; Macular Degeneration, Age-related, 3; Cutis Laxa, Autosomal Recessive, Type Ia