Porcine Protectin ELISA Kit | CD59 elisa kit

Porcine Protectin ELISA Kit

Cat. Num. AAA739174

• Gene Names: CD59; 1F5; EJ16; EJ30; EL32; G344; MIN1; MIN2; MIN3; MIRL; HRF20; MACIF; MEM43; MIC11; MSK21; 16.3A5; HRF-20; MAC-IP; p18-20
• Reactivity: Porcine
• Synonyms: Protectin; Porcine Protectin ELISA Kit; CD59 elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.
• Intended Uses: This CD 59 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine CD 59. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CD59 elisa kit

Principle of the Assay: CD 59 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CD 59 antibody and an CD 59-HRP conjugate. The assay sample and buffer are incubated together with CD 59-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD 59 concentration since CD 59 from samples and CD 59-HRP conjugate compete for the anti-CD 59 antibody binding site. Since the number of sites is limited, as more sites are occupied by CD 59 from the sample, fewer sites are left to bind CD 59-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD 59 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CD59 elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 42716299
• NCBI GeneID: 966
• NCBI Accession #: NP_976076.1
• NCBI GenBank Nucleotide #: NM_203331.2
• Molecular Weight: 14,177 Da
• NCBI Official Full Name: CD59 glycoprotein preproprotein
• NCBI Official Synonym Full Names: CD59 molecule, complement regulatory protein
• NCBI Official Symbol: CD59
• NCBI Official Synonym Symbols: 1F5; EJ16; EJ30; EL32; G344; MIN1; MIN2; MIN3; MIRL; HRF20; MACIF; MEM43; MIC11; MSK21; 16.3A5; HRF-20; MAC-IP; p18-20
• NCBI Protein Information: CD59 glycoprotein; protectin; 1F5 antigen; MEM43 antigen; Ly-6-like protein; T cell-activating protein; human leukocyte antigen MIC11; lymphocytic antigen CD59/MEM43; 20 kDa homologous restriction factor; membrane inhibitor of reactive lysis; membrane attack complex inhibition factor; membrane attack complex (MAC) inhibition factor; surface anitgen recognized by monoclonal antibody 16.3A5; CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344)
• UniProt Protein Name: CD59 glycoprotein
• Protein Family: CD59 glycoprotein
• UniProt Gene Name: CD59
• UniProt Synonym Gene Names: MIC11; MIN1; MIN2; MIN3; MSK21; HRF-20; HRF20; MAC-IP; MACIF; MIRL
• UniProt Entry Name: CD59_HUMAN

NCBI Description

This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. This protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CD59: Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. Defects in CD59 are the cause of CD59 deficiency (CD59D).

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 11p13

Cellular Component: anchored to external side of plasma membrane; extracellular space; compact myelin; focal adhesion; cell surface; membrane; plasma membrane; extracellular region; vesicle; sarcolemma

Molecular Function: complement binding; protein binding

Biological Process: cell activation; cell surface receptor linked signal transduction; regulation of complement activation; negative regulation of activation of membrane attack complex; innate immune response; positive regulation of T cell proliferation; blood coagulation; negative regulation of apoptosis

Disease: Hemolytic Anemia, Cd59-mediated, With Or Without Immune-mediated Polyneuropathy