Canine S100 Calcium Binding Protein A11 ELISA Kit | S100A11 elisa kit

Canine S100 Calcium Binding Protein A11 ELISA Kit

Cat. Num. AAA738727

• Gene Names: S100A11; MLN70; S100C; HEL-S-43
• Reactivity: Canine
• Synonyms: S100 Calcium Binding Protein A11; Canine S100 Calcium Binding Protein A11 ELISA Kit; S100A11 elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 2.5-50ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This S100A11 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine S100A11. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for S100A11 elisa kit

Principle of the Assay: S100A11 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-S100A11 antibody and an S100A11-HRP conjugate. The assay sample and buffer are incubated together with S100A11-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the S100A11 concentration since S100A11 from samples and S100A11-HRP conjugate compete for the anti-S100A11 antibody binding site. Since the number of sites is limited, as more sites are occupied by S100A11 from the sample, fewer sites are left to bind S100A11-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The S100A11 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for S100A11 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 119573777
• NCBI GeneID: 6282
• Molecular Weight: 11,740 Da
• NCBI Official Full Name: S100 calcium binding protein A11 (calgizzarin)
• NCBI Official Synonym Full Names: S100 calcium binding protein A11
• NCBI Official Symbol: S100A11
• NCBI Official Synonym Symbols: MLN70; S100C; HEL-S-43
• NCBI Protein Information: protein S100-A11; MLN 70; calgizzarin; protein S100-C; epididymis secretory protein Li 43; metastatic lymph node gene 70 protein; S100 calcium-binding protein A11 (calgizzarin)
• UniProt Protein Name: Protein S100-A11
• Protein Family: Protein
• UniProt Gene Name: S100A11
• UniProt Synonym Gene Names: MLN70; S100C; MLN 70
• UniProt Entry Name: S10AB_HUMAN

NCBI Description

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. [provided by RefSeq, Jul 2008]

Uniprot Description

S100A11: a calcium-binding regulatory protein of the S-100 family. Interacts with the N-termini of annexins A1. May regulate fusion processes such as endo- and exocytosis. Increases in the early stage of pancreatic carcinogenesis and decreases during subsequent progression to cancer. Increased expression in anaplastic large cell lymphoma.

Protein type: Cell cycle regulation; Calcium-binding; DNA replication

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: nucleoplasm; ruffle; extracellular space; cytoplasm; nucleus

Molecular Function: protein binding; protein homodimerization activity; calcium ion binding; calcium-dependent protein binding

Biological Process: negative regulation of cell proliferation; negative regulation of DNA replication; signal transduction