Typical Testing Data/Standard Curve (for reference only)
Sheep Dickkopf Related Protein 2 ELISA Kit | DKK2 elisa kit
Sheep Dickkopf Related Protein 2 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
INTENDED USE This DKK2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep DKK2. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY DKK2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DKK2 antibody and an DKK2-HRP conjugate. The assay sample and buffer are incubated together with DKK2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DKK2 concentration since DKK2 from samples and DKK2-HRP conjugate compete for the anti-DKK2 antibody binding site. Since the number of sites is limited, as more sites are occupied by DKK2 from the sample, fewer sites are left to bind DKK2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DKK2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. It can act as either an agonist or antagonist of Wnt/beta-catenin signaling, depending on the cellular context and the presence of the co-factor kremen 2. Activity of this protein is also modulated by binding to the Wnt co-receptor LDL-receptor related protein 6 (LRP6). [provided by RefSeq, Jul 2008]
Uniprot Description
DKK2: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Interacts with LRP5 and LRP6. Expressed in heart, brain, skeletal muscle and lung. Belongs to the dickkopf family.
Protein type: Secreted, signal peptide; Inhibitor; Secreted
Chromosomal Location of Human Ortholog: 4q25
Cellular Component: extracellular space
Biological Process: Wnt receptor signaling pathway; multicellular organismal development
Research Articles on DKK2
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Product Notes
The Sheep DKK2 dkk2 (Catalog #AAA738364) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA738364 ELISA Kit recognizes Sheep DKK2. It is sometimes possible for the material contained within the vial of "Dickkopf Related Protein 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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