Porcine Bone Morphogenetic Protein 15 ELISA Kit | BMP15 elisa kit

Porcine Bone Morphogenetic Protein 15 ELISA Kit

Cat. Num. AAA737825

• Gene Names: BMP15; ODG2; POF4; GDF9B
• Reactivity: Porcine
• Synonyms: Bone Morphogenetic Protein 15; Porcine Bone Morphogenetic Protein 15 ELISA Kit; BMP15 elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This BMP15 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine BMP15. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BMP15 elisa kit

Principle of the Assay: BMP15 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BMP15 antibody and an BMP15-HRP conjugate. The assay sample and buffer are incubated together with BMP15-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BMP15 concentration since BMP15 from samples and BMP15-HRP conjugate compete for the anti-BMP15 antibody binding site. Since the number of sites is limited, as more sites are occupied by BMP15 from the sample, fewer sites are left to bind BMP15-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BMP15 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for BMP15 elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 257743454
• NCBI GeneID: 9210
• NCBI Accession #: NP_005439.2
• NCBI GenBank Nucleotide #: NM_005448.2
• Molecular Weight: 45,055 Da
• NCBI Official Full Name: bone morphogenetic protein 15
• NCBI Official Synonym Full Names: bone morphogenetic protein 15
• NCBI Official Symbol: BMP15
• NCBI Official Synonym Symbols: ODG2; POF4; GDF9B
• NCBI Protein Information: bone morphogenetic protein 15; BMP-15; GDF-9B; growth/differentiation factor 9B
• UniProt Protein Name: Bone morphogenetic protein 15
• Protein Family: Bone morphogenetic protein
• UniProt Gene Name: BMP15
• UniProt Synonym Gene Names: GDF9B; BMP-15; GDF-9B
• UniProt Entry Name: BMP15_HUMAN

NCBI Description

The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The transforming growth factor-beta superfamily includes large families of growth and differentiation factors. It is thought that this protein may be involved in oocyte maturation and follicular development as a homodimer or by forming heterodimers with a related protein, Gdf9. Defects in this gene are the cause of ovarian dysgenesis 2.[provided by RefSeq, Sep 2009]

Uniprot Description

BMP15: May be involved in follicular development. Oocyte- specific growth/differentiation factor that stimulates folliculogenesis and granulosa cell (GC) growth. Defects in BMP15 are the cause of ovarian dysgenesis type 2 (ODG2); also known as X-linked hypergonadotropic ovarian dysgenesis or hypergonadotropic ovarian failure due to ovarian dysgenesis. Ovarian dysgenesis leads to ovarian failure and accounts for about half of the cases of primary amenorrhea. Defects in BMP15 are the cause of premature ovarian failure type 4 (POF4). An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol. Belongs to the TGF-beta family.

Protein type: Cytokine; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: Xp11.2

Cellular Component: extracellular space; cytoplasm

Molecular Function: growth factor activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: regulation of apoptosis; BMP signaling pathway; ovarian follicle development; granulosa cell development; positive regulation of transcription, DNA-dependent; regulation of MAPKKK cascade; female gamete generation; cell development

Disease: Ovarian Dysgenesis 2