Sheep Pleckstrin Homology Like Domain Family A Member 2 ELISA Kit | PHLDA2 elisa kit

Sheep Pleckstrin Homology Like Domain Family A Member 2 ELISA Kit

Cat. Num. AAA737499

• Gene Names: PHLDA2; IPL; BRW1C; BWR1C; HLDA2; TSSC3
• Reactivity: Sheep
• Synonyms: Pleckstrin Homology Like Domain Family A Member 2; Sheep Pleckstrin Homology Like Domain Family A Member 2 ELISA Kit; PHLDA2 elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This PHLDA2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep PHLDA2. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PHLDA2 elisa kit

Principle of the Assay: PHLDA2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PHLDA2. Standards or samples are then added to the microtiter plate wells and PHLDA2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PHLDA2 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for PHLDA2 are added to each well to "sandwich" the PHLDA2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PHLDA2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PHLDA2 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for PHLDA2 elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 4507705
• NCBI GeneID: 7262
• NCBI Accession #: NP_003302.1
• NCBI GenBank Nucleotide #: NM_003311.3
• Molecular Weight: 17,092 Da
• NCBI Official Full Name: pleckstrin homology-like domain family A member 2
• NCBI Official Synonym Full Names: pleckstrin homology-like domain, family A, member 2
• NCBI Official Symbol: PHLDA2
• NCBI Official Synonym Symbols: IPL; BRW1C; BWR1C; HLDA2; TSSC3
• NCBI Protein Information: pleckstrin homology-like domain family A member 2; p17-BWR1C; tumor-supressing STF cDNA 3; p17-Beckwith-Wiedemann region 1C; p17-Beckwith-Wiedemann region 1 C; tumor-suppressing STF cDNA 3 protein; imprinted in placenta and liver protein; tumor suppressing subtransferable candidate 3; tumor suppressing subchromosomal transferable fragment cDNA 3; beckwith-Wiedemann syndrome chromosomal region 1 candidate gene C protein; tumor-suppressing subchromosomal transferable fragment candidate gene 3 protein
• UniProt Protein Name: Pleckstrin homology-like domain family A member 2
• Protein Family: Pleckstrin homology-like domain family
• UniProt Gene Name: PHLDA2
• UniProt Synonym Gene Names: BWR1C; HLDA2; IPL; TSSC3; p17-BWR1C
• UniProt Entry Name: PHLA2_HUMAN

NCBI Description

This gene is located in a cluster of imprinted genes on chromosome 11p15.5, which is considered to be an important tumor suppressor gene region. Alterations in this region may be associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. This gene has been shown to be imprinted, with preferential expression from the maternal allele in placenta and liver. [provided by RefSeq, Oct 2010]

Uniprot Description

PHLDA2: a protein may play a role in regulating placenta growth. Contains one PH domain. Expressed in placenta and adult prostate gland. Expressed at low levels in adult liver and lung, and fetal liver. Expressed in adult brain and neuroblastoma, medullablastoma and glioblastoma cell lines.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: membrane; cytoplasm

Biological Process: organ morphogenesis; apoptosis; regulation of gene expression; regulation of embryonic development; regulation of cell migration; placenta development