Canine Pepsinogen A ELISA Kit | PGA elisa kit

Canine Pepsinogen A ELISA Kit

Cat. Num. AAA735622

• Reactivity: Canine
• Synonyms: Pepsinogen A; Canine Pepsinogen A ELISA Kit; PGA elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 50-1000pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This PGA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine PGA. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PGA elisa kit

Principle of the Assay: PGA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PGA antibody and an PGA-HRP conjugate. The assay sample and buffer are incubated together with PGA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PGA concentration since PGA from samples and PGA-HRP conjugate compete for the anti-PGA antibody binding site. Since the number of sites is limited, as more sites are occupied by PGA from the sample, fewer sites are left to bind PGA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PGA concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 387013
• NCBI GeneID: 5222
• Molecular Weight: 41,993 Da
• NCBI Official Full Name: pepsinogen A
• NCBI Official Synonym Full Names: pepsinogen 5, group I (pepsinogen A)
• NCBI Official Symbol: PGA5
• NCBI Protein Information: pepsin A-5; pepsinogen-5; pepsinogen A5
• UniProt Protein Name: Pepsin A-5
• Protein Family: Pepsin
• UniProt Gene Name: PGA5
• UniProt Entry Name: PEPA5_HUMAN

NCBI Description

This gene encodes a protein precursor of the digestive enzyme pepsin, a member of the peptidase A1 family of endopeptidases. The encoded precursor is secreted by gastric chief cells and undergoes autocatalytic cleavage in acidic conditions to form the active enzyme, which functions in the digestion of dietary proteins. This gene is found in a cluster of related genes on chromosome 11, each of which encodes one of multiple pepsinogens. Pepsinogen levels in serum may serve as a biomarker for atrophic gastritis and gastric cancer. [provided by RefSeq, Jul 2015]

Uniprot Description

PGA5: Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. Belongs to the peptidase A1 family.

Protein type: EC 3.4.23.1; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11q13

Molecular Function: aspartic-type endopeptidase activity

Biological Process: digestion; proteolysis