Monkey Peroxisome proliferators activator alpha ELISA Kit | PPAR-alpha elisa kit

Monkey Peroxisome proliferators activator alpha ELISA Kit

Cat. Num. AAA734246

• Gene Names: PPARA; PPAR; NR1C1; hPPAR; PPARalpha
• Reactivity: Monkey
• Synonyms: Peroxisome proliferators activator alpha; Monkey Peroxisome proliferators activator alpha ELISA Kit; Monkey Peroxisome proliferators activator a ELISA Kit; PPAR-alpha elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This PPAR-? ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey PPAR-?. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PPAR-alpha elisa kit

Principle of the assay: PPAR-? ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PPAR-? antibody and an PPAR-?-HRP conjugate. The assay sample and buffer are incubated together with PPAR-?-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PPAR-? concentration since PPAR-? from samples and PPAR-?-HRP conjugate compete for the anti-PPAR-? antibody binding site. Since the number of sites is limited, as more sites are occupied by PPAR-? from the sample, fewer sites are left to bind PPAR-?-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PPAR-? concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 50348666
• NCBI GeneID: 5465
• NCBI Accession #: NP_001001928.1
• NCBI GenBank Nucleotide #: NM_001001928.2
• Molecular Weight: 18,942 Da
• NCBI Official Full Name: peroxisome proliferator-activated receptor alpha
• NCBI Official Synonym Full Names: peroxisome proliferator-activated receptor alpha
• NCBI Official Symbol: PPARA
• NCBI Official Synonym Symbols: PPAR; NR1C1; hPPAR; PPARalpha
• NCBI Protein Information: peroxisome proliferator-activated receptor alpha; PPAR-alpha; nuclear receptor subfamily 1 group C member 1; peroxisome proliferative activated receptor, alpha; peroxisome proliferator-activated nuclear receptor alpha variant 3
• UniProt Protein Name: Peroxisome proliferator-activated receptor alpha
• UniProt Gene Name: PPARA
• UniProt Synonym Gene Names: NR1C1; PPAR; PPAR-alpha
• UniProt Entry Name: PPARA_HUMAN

NCBI Description

Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

PPAR-alpha: Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl- 2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. Belongs to the nuclear hormone receptor family. NR1 subfamily.

Protein type: DNA-binding; Nuclear receptor

Chromosomal Location of Human Ortholog: 22q13.31

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein domain specific binding; ligand-dependent nuclear receptor activity; NFAT protein binding; zinc ion binding; drug binding; phosphatase binding; protein binding; DNA binding; ubiquitin conjugating enzyme binding; sequence-specific DNA binding; protein complex binding; steroid hormone receptor activity; lipid binding; transcription factor activity

Biological Process: circadian rhythm; epidermis development; wound healing; positive regulation of transcription, DNA-dependent; heart development; behavioral response to nicotine; negative regulation of transcription from RNA polymerase II promoter; cellular lipid metabolic process; fatty acid metabolic process; negative regulation of appetite; response to insulin stimulus; positive regulation of gluconeogenesis; circadian regulation of gene expression; negative regulation of blood pressure; negative regulation of protein binding; negative regulation of glycolysis; transcription initiation from RNA polymerase II promoter; intracellular receptor-mediated signaling pathway; lipoprotein metabolic process; regulation of circadian rhythm; positive regulation of fatty acid beta-oxidation; positive regulation of fatty acid oxidation; response to hypoxia; steroid hormone mediated signaling; gene expression; positive regulation of transcription from RNA polymerase II promoter; lipid metabolic process; fatty acid transport