Canine Neuroptide Y ELISA Kit | NP-Y elisa kit

Canine Neuroptide Y ELISA Kit

Cat. Num. AAA734092

• Gene Names: NPY; PYY4
• Reactivity: Canine
• Synonyms: Neuroptide Y; Canine Neuroptide Y ELISA Kit; NP-Y elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 250-5000pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This NP-Y ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine NP-Y. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NP-Y elisa kit

Principle of the Assay: NP-Y ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NP-Y antibody and an NP-Y-HRP conjugate. The assay sample and buffer are incubated together with NP-Y-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NP-Y concentration since NP-Y from samples and NP-Y-HRP conjugate compete for the anti-NP-Y antibody binding site. Since the number of sites is limited, as more sites are occupied by NP-Y from the sample, fewer sites are left to bind NP-Y-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NP-Y concentration in each sample is interpolated from this standard curve.

Product Categories/Family for NP-Y elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 189274
• NCBI GeneID: 4852
• Molecular Weight: 10,851 Da
• NCBI Official Full Name: neuropeptide Y
• NCBI Official Synonym Full Names: neuropeptide Y
• NCBI Official Symbol: NPY
• NCBI Official Synonym Symbols: PYY4
• NCBI Protein Information: pro-neuropeptide Y; prepro-neuropeptide Y
• UniProt Protein Name: Pro-neuropeptide Y
• UniProt Gene Name: NPY
• UniProt Synonym Gene Names: NPY; CPON
• UniProt Entry Name: NPY_HUMAN

NCBI Description

This gene encodes a neuropeptide that is widely expressed in the central nervous system and influences many physiological processes, including cortical excitability, stress response, food intake, circadian rhythms, and cardiovascular function. The neuropeptide functions through G protein-coupled receptors to inhibit adenylyl cyclase, activate mitogen-activated protein kinase (MAPK), regulate intracellular calcium levels, and activate potassium channels. A polymorphism in this gene resulting in a change of leucine 7 to proline in the signal peptide is associated with elevated cholesterol levels, higher alcohol consumption, and may be a risk factor for various metabolic and cardiovascular diseases. [provided by RefSeq, Nov 2009]

Uniprot Description

NPY: NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone. Belongs to the NPY family.

Protein type: Secreted, signal peptide; Secreted; Hormone

Chromosomal Location of Human Ortholog: 7p15.1

Cellular Component: Golgi apparatus; extracellular space; cell; extracellular region

Molecular Function: G-protein coupled receptor activity; neuropeptide hormone activity; neuropeptide Y receptor binding; calcium channel regulator activity; receptor binding

Biological Process: blood circulation; behavior; adult feeding behavior; synaptic transmission; G-protein signaling, coupled to cyclic nucleotide second messenger; cell proliferation; neuropeptide signaling pathway; calcium ion transport; positive regulation of appetite; regulation of blood pressure; regulation of appetite; digestion; feeding behavior; cerebral cortex development; cell motility; neurite development; central nervous system neuron development