Rat Anti-Intrinsic Factor Antibody ELISA Kit | IF elisa kit

Rat Anti-Intrinsic Factor Antibody ELISA Kit

Cat. Num. AAA732425

• Reactivity: Rat
• Synonyms: Anti-Intrinsic Factor Antibody; Rat Anti-Intrinsic Factor Antibody ELISA Kit; IF elisa kit

• Reactivity: Rat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0-250 ng/mL
• Sensitivity: 1.0 ng/mL
• Intended Uses: This IFAb ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat IFAb. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IF elisa kit

Principle of the assay: IFAb ELISA kit applies the competitive enzyme immunoassay technique utilizing Intrinsic Factor antigen and an IFAb-HRP conjugate. The assay sample and buffer are incubated together with IFAb-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IFAb concentration since IFAb from samples and IFAb-HRP conjugate compete for the Intrinsic Factor antigen binding site. Since the number of sites is limited, as more sites are occupied by IFAb from the sample, fewer sites are left to bind IFAb-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IFAb concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 74996854
• NCBI GeneID: 8624902
• Molecular Weight: 45,761 Da
• NCBI Official Full Name: Apoptosis-inducing factor homolog A
• NCBI Official Symbol: aifC
• NCBI Protein Information: hypothetical protein
• UniProt Protein Name: Apoptosis-inducing factor homolog A
• UniProt Gene Name: aifA
• UniProt Synonym Gene Names: DDB_G0285003
• UniProt Entry Name: AIFA_DICDI

Uniprot Description

Putative FAD-dependent oxidoreductase.