Human Lactoferrin ELISA Kit | LTF/LF elisa kit

Human Lactoferrin ELISA Kit

Cat. Num. AAA731129

• Gene Names: LTF; LF; HLF2; GIG12
• Reactivity: Human
• Synonyms: Lactoferrin; Human Lactoferrin ELISA Kit; Lactoferrin (Human); LTF/LF elisa kit

• Reactivity: Human

• Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LTF/LF elisa kit

For Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma

Intended Uses: This LTF/LF ELISA kit is intended for laboratory research use only. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of LTF/LF in the sample, this LTF/LF ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus LTF/LF concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of LTF/LF utilizes a multiclonal anti-LTF/LF antibody and an LTF/LF-HRP conjugate. The assay sample and buffer are incubated together with LTF/LF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LTF/LF concentration since LTF/LF from samples and LTF/LF-HRP conjugate compete for the anti-LTF/LF antibody binding site. Since the number of sites is limited, as more sites are occupied by LTF/LF from the sample, fewer sites are left to bind LTF/LF-HRP conjugate. Standards of known LTF/LF concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of LTF/LF. The LTF/LF concentration in each sample is interpolated from this standard curve.

Product Categories/Family for LTF/LF elisa kit

Human ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 312433998
• NCBI GeneID: 4057
• NCBI Accession #: NP_001186078.1
• NCBI GenBank Nucleotide #: NM_001199149.1
• UniProt Accession #: P02788; O00756; Q16780; Q16785; Q16786; Q16789; Q8IU92; Q8IZH6; Q8TCD2; Q96KZ4; Q96KZ5; Q9H1Z3; Q19KS2; Q2TUW9; Q5DSM0; Q5EK51; Q8IWS6; Q8IX02
• Molecular Weight: 78,182 Da
• NCBI Official Full Name: lactotransferrin isoform 2
• NCBI Official Synonym Full Names: lactotransferrin
• NCBI Official Symbol: LTF
• NCBI Official Synonym Symbols: LF; HLF2; GIG12
• NCBI Protein Information: lactotransferrin; talalactoferrin; OTTHUMP00000209779; OTTHUMP00000209780; OTTHUMP00000209781; neutrophil lactoferrin; growth-inhibiting protein 12
• UniProt Protein Name: Lactotransferrin
• Protein Family: Lactoferrin-binding protein
• UniProt Gene Name: LTF
• UniProt Synonym Gene Names: LF
• UniProt Entry Name: TRFL_HUMAN

NCBI Description

This gene is a member of the transferrin family of genes and its protein product is found in the secondary granules of neutrophils. The protein is a major iron-binding protein in milk and body secretions with an antimicrobial activity, making it an important component of the non-specific immune system. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Uniprot Description

LTF: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. Belongs to the transferrin family. 2 isoforms of the human protein are produced by alternative promoter.

Protein type: Secreted, signal peptide; Protease; Secreted; EC 3.4.21.-

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: extracellular space; specific granule; cytoplasm; extracellular region; nucleus; secretory granule

Molecular Function: heparin binding; protein serine/threonine kinase activator activity; protein binding; ferric iron binding; DNA binding; iron ion binding; serine-type endopeptidase activity

Biological Process: interaction with host; negative regulation of lipopolysaccharide-mediated signaling pathway; ossification; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; positive regulation of toll-like receptor 4 signaling pathway; antifungal humoral response; proteolysis; iron ion transport; humoral immune response; regulation of cytokine production; activation of NF-kappaB transcription factor; positive regulation of osteoblast differentiation; retinal homeostasis; negative regulation of viral genome replication; innate immune response in mucosa; response to host immune response; positive regulation of osteoblast proliferation; antibacterial humoral response; regulation of tumor necrosis factor production; negative regulation of ATPase activity; iron assimilation by chelation and transport; negative regulation of viral reproduction; negative regulation of apoptosis