Guinea Pig Angiopoietin 4 ELISA Kit | ANG4 elisa kit

Guinea pig Angiopoietin 4 ELISA Kit

Cat. Num. AAA730707

• Gene Names: ANGPT4; AGP4; ANG4; ANG-3
• Reactivity: Guinea Pig
• Synonyms: Angiopoietin 4; Guinea pig Angiopoietin 4 ELISA Kit; ANG4 elisa kit

• Reactivity: Guinea Pig

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This ANG4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig ANG4. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ANG4 elisa kit

Principle of the assay: ANG4 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ANG4. Standards or samples are then added to the microtiter plate wells and ANG4 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ANG4 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ANG4 are added to each well to “sandwich” the ANG4 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ANG4 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANG4 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ANG4 elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 7705276
• NCBI GeneID: 51378
• NCBI Accession #: NP_057069.1
• NCBI GenBank Nucleotide #: NM_015985.2
• Molecular Weight: 46,416 Da
• NCBI Official Full Name: angiopoietin-4
• NCBI Official Synonym Full Names: angiopoietin 4
• NCBI Official Symbol: ANGPT4
• NCBI Official Synonym Symbols: AGP4; ANG4; ANG-3
• NCBI Protein Information: angiopoietin-4; ANG-4; angiopoietin-3; dJ824F16.2 (angiopoietin 4)
• UniProt Protein Name: Angiopoietin-4
• Protein Family: Angiogenin
• UniProt Gene Name: ANGPT4
• UniProt Synonym Gene Names: ANG3; ANG4; ANG-4; ANG-3
• UniProt Entry Name: ANGP4_HUMAN

NCBI Description

Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The mechanism by which they contribute to angiogenesis is thought to involve regulation of endothelial cell interactions with supporting perivascular cells. The protein encoded by this gene functions as an agonist and is an angiopoietin. [provided by RefSeq, Jul 2008]

Uniprot Description

ANGPT4: Binds to TEK/TIE2, modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2. Promotes endothelial cell survival, migration and angiogenesis.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: extracellular space; extracellular region

Molecular Function: transmembrane receptor protein tyrosine kinase activator activity; receptor tyrosine kinase binding

Biological Process: positive regulation of angiogenesis; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of angiogenesis; transmembrane receptor protein tyrosine kinase activation (dimerization); endothelial cell proliferation; positive regulation of blood vessel endothelial cell migration; angiogenesis; blood coagulation; negative regulation of blood vessel endothelial cell migration; leukocyte migration; negative regulation of apoptosis