Typical Testing Data/Standard Curve (for reference only)
Human Insulin Like Growth Factor Binding Protein 2 ELISA Kit | IGFBP2 elisa kit
Human Insulin Like Growth Factor Binding Protein 2 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: IGFBP-2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for IGFBP-2. Standards or samples are then added to the microtiter plate wells and IGFBP-2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IGFBP-2 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for IGFBP-2 are added to each well to "sandwich" the IGFBP-2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IGFBP-2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IGFBP-2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
Uniprot Description
IGFBP2: Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Binds IGF2 more than IGF1.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 2q35
Cellular Component: extracellular space; apical plasma membrane; extracellular region; cytoplasmic vesicle
Molecular Function: protein binding; insulin-like growth factor I binding; insulin-like growth factor II binding
Biological Process: response to drug; response to retinoic acid; regulation of insulin-like growth factor receptor signaling pathway; response to lithium ion; response to glucocorticoid stimulus; female pregnancy; positive regulation of activated T cell proliferation; signal transduction; response to estradiol stimulus; cellular response to hormone stimulus; cellular protein metabolic process; response to mechanical stimulus; regulation of cell growth; response to nutrient; aging
Research Articles on IGFBP2
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Product Notes
The Human IGFBP2 igfbp2 (Catalog #AAA730345) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA730345 ELISA Kit recognizes Human IGFBP2. It is sometimes possible for the material contained within the vial of "Insulin Like Growth Factor Binding Protein 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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