Rat CCAAT/Enhancer Binding Protein Gamma ELISA Kit | CEBPgamma elisa kit

Rat CCAAT/Enhancer Binding Protein Gamma ELISA Kit

Cat. Num. AAA729477

• Gene Names: CEBPG; GPE1BP; IG/EBP-1
• Reactivity: Rat
• Synonyms: CCAAT/Enhancer Binding Protein Gamma; Rat CCAAT/Enhancer Binding Protein Gamma ELISA Kit; CEBPgamma elisa kit

• Reactivity: Rat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This CEBP? ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat CEBP?. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CEBPgamma elisa kit

Principle of the assay: CEBP? ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CEBP? antibody and an CEBP?-HRP conjugate. The assay sample and buffer are incubated together with CEBP?-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CEBP? concentration since CEBP? from samples and CEBP?-HRP conjugate compete for the anti-CEBP? antibody binding site. Since the number of sites is limited, as more sites are occupied by CEBP? from the sample, fewer sites are left to bind CEBP?-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CEBP? concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CEBPgamma elisa kit

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 356582519
• NCBI GeneID: 1054
• NCBI Accession #: NP_001239225.1
• NCBI GenBank Nucleotide #: NM_001252296.1
• Molecular Weight: 16,408 Da
• NCBI Official Full Name: CCAAT/enhancer-binding protein gamma
• NCBI Official Synonym Full Names: CCAAT/enhancer binding protein (C/EBP), gamma
• NCBI Official Symbol: CEBPG
• NCBI Official Synonym Symbols: GPE1BP; IG/EBP-1
• NCBI Protein Information: CCAAT/enhancer-binding protein gamma; c/EBP gamma
• UniProt Protein Name: CCAAT/enhancer-binding protein gamma
• UniProt Gene Name: CEBPG
• UniProt Synonym Gene Names: C/EBP gamma
• UniProt Entry Name: CEBPG_HUMAN

NCBI Description

The C/EBP family of transcription factors regulates viral and cellular CCAAT/enhancer element-mediated transcription. C/EBP proteins contain the bZIP region, which is characterized by two motifs in the C-terminal half of the protein: a basic region involved in DNA binding and a leucine zipper motif involved in dimerization. The C/EBP family consist of several related proteins, C/EBP alpha, C/EBP beta, C/EBP gamma, and C/EBP delta, that form homodimers and that form heterodimers with each other. CCAAT/enhancer binding protein gamma may cooperate with Fos to bind PRE-I enhancer elements. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

C/EBP-gamma: Transcription factor that binds to the enhancer element PRE-I (positive regulatory element-I) of the IL-4 gene. Might change the DNA-binding specificity of other transcription factors and recruit them to unusual DNA sites. Belongs to the bZIP family. C/EBP subfamily.

Protein type: DNA-binding; Motility/polarity/chemotaxis; Transcription factor

Chromosomal Location of Human Ortholog: 19q13.11

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; double-stranded DNA binding; transcription factor binding

Biological Process: regulation of transcription from RNA polymerase II promoter; positive regulation of DNA repair; transcription, DNA-dependent; natural killer cell mediated cytotoxicity; B cell differentiation; negative regulation of transcription factor activity; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; immune response; enucleate erythrocyte differentiation; liver development; mRNA metabolic process; positive regulation of interferon-gamma biosynthetic process; positive regulation of DNA binding