Mouse Complement fragment 4a ELISA Kit | C4a elisa kit

Mouse Complement fragment 4a ELISA Kit

Cat. Num. AAA728156

• Gene Names: C4A; C4; RG; C4S; CO4; C4A2; C4A3; C4A4; C4A6; C4AD; CPAMD2
• Reactivity: Mouse
• Synonyms: Complement fragment 4a; Mouse Complement fragment 4a ELISA Kit; C4a elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 2.5-50ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This C4a ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse C4a. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for C4a elisa kit

Principle of the Assay: C4a ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-C4a antibody and an C4a-HRP conjugate. The assay sample and buffer are incubated together with C4a-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the C4a concentration since C4a from samples and C4a-HRP conjugate compete for the anti-C4a antibody binding site. Since the number of sites is limited, as more sites are occupied by C4a from the sample, fewer sites are left to bind C4a-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C4a concentration in each sample is interpolated from this standard curve.

Product Categories/Family for C4a elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 67190748
• NCBI GeneID: 720
• NCBI Accession #: NP_009224.2
• NCBI GenBank Nucleotide #: NM_007293.2
• Molecular Weight: 187,704 Da
• NCBI Official Full Name: complement C4-A isoform 1 preproprotein
• NCBI Official Synonym Full Names: complement component 4A (Rodgers blood group)
• NCBI Official Symbol: C4A
• NCBI Official Synonym Symbols: C4; RG; C4S; CO4; C4A2; C4A3; C4A4; C4A6; C4AD; CPAMD2
• NCBI Protein Information: complement C4-A; acidic C4; C4A anaphylatoxin; Rodgers form of C4; acidic complement C4; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2
• UniProt Protein Name: Complement C4-A
• Protein Family: Complement C4
• UniProt Gene Name: C4A
• UniProt Synonym Gene Names: CO4; CPAMD2
• UniProt Entry Name: CO4A_HUMAN

NCBI Description

This gene encodes the acidic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain may be cleaved to release C4 anaphylatoxin, a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus and type I diabetes mellitus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

C4B: C4 plays a central role in the activation of the classical pathway of the complement system. It is processed by activated C1 which removes from the alpha chain the C4a anaphylatoxin. The remaining alpha chain fragment C4b is the major activation product and is an essential subunit of the C3 convertase (C4b2a) and the C5 convertase (C3bC4b2a) enzymes of the classical complement pathway. Defects in C4B are a cause of susceptibility to systemic lupus erythematosus (SLE). A chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Interindividual copy- number variation (CNV) of complement component C4 and associated polymorphisms result in different susceptibilities to SLE. The risk of SLE susceptibility has been shown to be significantly increased among subjects with only two copies of total C4. A high copy number is a protective factor against SLE. Defects in C4B are the cause of complement component 4B deficiency (C4BD). A rare defect of the complement classical pathway associated with the development of autoimmune disorders, mainly systemic lupus with or without associated glomerulonephritis.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: extracellular space; extracellular region; plasma membrane

Molecular Function: complement binding; endopeptidase inhibitor activity; carbohydrate binding

Biological Process: detection of molecule of bacterial origin; regulation of complement activation; innate immune response; opsonization; inflammatory response; complement activation, classical pathway; complement activation

Disease: Complement Component 4b Deficiency