Human Creatine Kinase ELISA Kit | CK elisa kit

Human Creatine Kinase ELISA Kit

Cat. Num. AAA727734

• Gene Names: CKMT1B; CKMT; CKMT1; UMTCK; CKMT1A
• Reactivity: Human
• Synonyms: Creatine Kinase; Human Creatine Kinase ELISA Kit; Human Creatine Kinase (CK) ELISA Kit; Creatine Kinase (Human); CK elisa kit

• Reactivity: Human

• Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CK elisa kit

Intended Uses: This CIZ1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CIZ1. This ELISA kit for research use only!

Principle of the Assay: CIZ1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CIZ1 antibody and an CIZ1-HRP conjugate. The assay sample and buffer are incubated together with CIZ1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CIZ1 concentration since CIZ1 from samples and CIZ1-HRP conjugate compete for the anti-CIZ1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CIZ1 from the sample, fewer sites are left to bind CIZ1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CIZ1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CK elisa kit

Human ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 180590
• NCBI GeneID: 1159
• UniProt Accession #: P12532; Q0VAM3; Q32NF6; Q53FC4; B4DIT8; B7ZA09
• Molecular Weight: 47,037 Da
• NCBI Official Full Name: creatine kinase
• NCBI Official Synonym Full Names: creatine kinase, mitochondrial 1B
• NCBI Official Symbol: CKMT1B
• NCBI Official Synonym Symbols: CKMT; CKMT1; UMTCK; CKMT1A
• NCBI Protein Information: creatine kinase U-type, mitochondrial; U-MtCK; mia-CK; OTTHUMP00000066275; OTTHUMP00000200125; ubiquitous mitochondrial creatine kinase; acidic-type mitochondrial creatine kinase; creatine kinase, mitochondrial 1 (ubiquitous)
• UniProt Protein Name: Creatine kinase U-type, mitochondrial
• Protein Family: Creatine kinase
• UniProt Gene Name: CKMT1A
• UniProt Synonym Gene Names: CKMT
• UniProt Entry Name: KCRU_HUMAN

NCBI Description

Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes located near each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins. [provided by RefSeq]

Uniprot Description

CKMT1A: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Belongs to the ATP:guanido phosphotransferase family. Exists as an octamer composed of four MTCK homodimers. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; EC 2.7.3.2; Amino Acid Metabolism - arginine and proline; Kinase, other

Chromosomal Location of Human Ortholog: 15q15

Cellular Component: mitochondrion; mitochondrial inner membrane

Molecular Function: creatine kinase activity; ATP binding

Biological Process: phosphorylation; creatine metabolic process