Rat Serine/Threonine-Protein Phosphatase PP1-alpha Catalytic Subunit ELISA Kit | PPP1CA elisa kit

Rat Serine/Threonine-Protein Phosphatase PP1-alpha Catalytic Subunit ELISA Kit

Cat. Num. AAA7255567

• Gene Names: PPP1CA; PP1A; PP-1A; PPP1A; PP1alpha
• Reactivity: Rat
• Synonyms: Serine/Threonine-Protein Phosphatase PP1-alpha Catalytic Subunit; Rat Serine/Threonine-Protein Phosphatase PP1-alpha Catalytic Subunit ELISA Kit; PPP1CA elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of sST2. No significant cross-reactivity or interference between sST2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between sST2 and all the analogues, therefore, cross reaction may still exist in some cases.
• Sequence Length: 330

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL
• Preparation and Storage: Store at 2-8 degree C.; The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for PPP1CA elisa kit

Intended Uses: This sST2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat sST2. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: sST2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-sST2 antibody and an sST2-HRP conjugate. The assay sample and buffer are incubated together with sST2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the sST2 concentration since sST2 from samples and sST2-HRP conjugate compete for the anti-sST2 antibody binding site. Since the number of sites is limited, as more sites are occupied by sST2 from the sample, fewer sites are left to bind sST2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sST2 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4506003
• NCBI GeneID: 5499
• NCBI Accession #: NP_002699.1
• NCBI GenBank Nucleotide #: NM_002708.4
• UniProt Accession #: P62136; P08129; P20653; P22802; Q07161; A6NNR3; B2R908
• Molecular Weight: 38,631 Da
• NCBI Official Full Name: serine/threonine-protein phosphatase PP1-alpha catalytic subunit isoform 1
• NCBI Official Synonym Full Names: protein phosphatase 1 catalytic subunit alpha
• NCBI Official Symbol: PPP1CA
• NCBI Official Synonym Symbols: PP1A; PP-1A; PPP1A; PP1alpha
• NCBI Protein Information: serine/threonine-protein phosphatase PP1-alpha catalytic subunit
• UniProt Protein Name: Serine/threonine-protein phosphatase PP1-alpha catalytic subunit
• Protein Family: Serine/threonine-protein phosphatase
• UniProt Gene Name: PPP1CA
• UniProt Synonym Gene Names: PPP1A; PP-1A

NCBI Description

The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Regulates NEK2 function in terms of kinase activity and centrosome number and splitting, both in the presence and absence of radiation-induced DNA damage. Regulator of neural tube and optic fissure closure, and enteric neural crest cell (ENCCs) migration during development. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E. Dephosphorylates the 'Ser-418' residue of FOXP3 in regulatory T-cells (Treg) from patients with rheumatoid arthritis, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Dephosphorylates CENPA (PubMed:25556658). Dephosphorylates the 'Ser-139' residue of ATG16L1 causing dissociation of ATG12-ATG5-ATG16L1 complex, thereby inhibiting autophagy (PubMed:26083323).