Typical Testing Data/Standard Curve (for reference only)
Human Transmembrane protein 119 ELISA Kit | TMEM119 elisa kit
Human Transmembrane protein 119 ELISA Kit
Gene Names
TMEM119; OBIF
Reactivity
Human
Synonyms
Transmembrane protein 119; Human Transmembrane protein 119 ELISA Kit; TMEM119 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of TMEM119. No significant cross-reactivity or interference between TMEM119 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TMEM119 and all the analogues, therefore, cross reaction may still exist in some cases.
Sequence Length
283
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for TMEM119 elisa kit
Intended Uses: This TMEM119 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human TMEM119. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: TMEM119 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-TMEM119 antibody and an TMEM119-HRP conjugate. The assay sample and buffer are incubated together with TMEM119-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TMEM119 concentration since TMEM119 from samples and TMEM119-HRP conjugate compete for the anti-TMEM119 antibody binding site. Since the number of sites is limited, as more sites are occupied by TMEM119 from the sample, fewer sites are left to bind TMEM119-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TMEM119 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: TMEM119 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-TMEM119 antibody and an TMEM119-HRP conjugate. The assay sample and buffer are incubated together with TMEM119-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TMEM119 concentration since TMEM119 from samples and TMEM119-HRP conjugate compete for the anti-TMEM119 antibody binding site. Since the number of sites is limited, as more sites are occupied by TMEM119 from the sample, fewer sites are left to bind TMEM119-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TMEM119 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
29,203 Da
NCBI Official Full Name
transmembrane protein 119
NCBI Official Synonym Full Names
transmembrane protein 119
NCBI Official Symbol
TMEM119
NCBI Official Synonym Symbols
OBIF
NCBI Protein Information
transmembrane protein 119
UniProt Protein Name
Transmembrane protein 119
Protein Family
UniProt Gene Name
TMEM119
UniProt Synonym Gene Names
OBIF
Uniprot Description
Plays an important role in bone formation and normal bone mineralization. Promotes the differentiation of myoblasts into osteoblasts (PubMed:20025746). May induce the commitment and differentiation of myoblasts into osteoblasts through an enhancement of BMP2 production and interaction with the BMP-RUNX2 pathway. Upregulates the expression of ATF4, a transcription factor which plays a central role in osteoblast differentiation. Essential for normal spermatogenesis and late testicular differentiation ().
Research Articles on TMEM119
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Product Notes
The Human TMEM119 tmem119 (Catalog #AAA7254556) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7254556 ELISA Kit recognizes Human TMEM119. It is sometimes possible for the material contained within the vial of "Transmembrane protein 119, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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