Human Endothelial Protein C Receptor ELISA Kit | EPCR elisa kit

Human Endothelial Protein C Receptor ELISA Kit

Cat. Num. AAA7254132

• Gene Names: PROCR; CCCA; EPCR; CCD41
• Reactivity: Human
• Synonyms: Endothelial Protein C Receptor; Human Endothelial Protein C Receptor ELISA Kit; EPCR elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of EPCR. No significant cross-reactivity or interference between EPCR and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between EPCR and all the analogues, therefore, cross reaction may still exist in some cases.
• Sequence Length: 238

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 1.0 ng/mL

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EPCR elisa kit

Intended Uses: This EPCR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human EPCR. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: EPCR ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-EPCR antibody and an EPCR-HRP conjugate. The assay sample and buffer are incubated together with EPCR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the EPCR concentration since EPCR from samples and EPCR-HRP conjugate compete for the anti-EPCR antibody binding site. Since the number of sites is limited, as more sites are occupied by EPCR from the sample, fewer sites are left to bind EPCR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EPCR concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 34335272
• NCBI GeneID: 10544
• NCBI Accession #: NP_006395.2
• NCBI GenBank Nucleotide #: NM_006404.4
• UniProt Accession #: Q9UNN8; Q14218; Q6IB56; Q96CB3; Q9ULX1; B2RC04
• Molecular Weight: 26,671 Da
• NCBI Official Full Name: endothelial protein C receptor
• NCBI Official Synonym Full Names: protein C receptor
• NCBI Official Symbol: PROCR
• NCBI Official Synonym Symbols: CCCA; EPCR; CCD41
• NCBI Protein Information: endothelial protein C receptor
• UniProt Protein Name: Endothelial protein C receptor
• Protein Family: Endothelial protein C receptor
• UniProt Gene Name: PROCR
• UniProt Synonym Gene Names: EPCR; APC receptor

NCBI Description

The protein encoded by this gene is a receptor for activated protein C, a serine protease activated by and involved in the blood coagulation pathway. The encoded protein is an N-glycosylated type I membrane protein that enhances the activation of protein C. Mutations in this gene have been associated with venous thromboembolism and myocardial infarction, as well as with late fetal loss during pregnancy. The encoded protein may also play a role in malarial infection and has been associated with cancer. [provided by RefSeq, Jul 2013]

Uniprot Description

Binds activated protein C. Enhances protein C activation by the thrombin-thrombomodulin complex; plays a role in the protein C pathway controlling blood coagulation.