Typical Testing Data/Standard Curve (for reference only)
Mouse Heme-binding protein 1 (HEBP1) ELISA Kit | HEBP1 elisa kit
Mouse Heme-binding protein 1 (HEBP1) ELISA Kit
Gene Names
HEBP1; HBP; HEBP
Reactivity
Mouse
Synonyms
Heme-binding protein 1 (HEBP1); Mouse Heme-binding protein 1 (HEBP1) ELISA Kit; HEBP1 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of HEBP1. No significant cross-reactivity or interference between HEBP1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HEBP1 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for HEBP1 elisa kit
Intended Uses: This HEBP1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse HEBP1. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: HEBP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-HEBP1 antibody and an HEBP1-HRP conjugate. The assay sample and buffer are incubated together with HEBP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HEBP1 concentration since HEBP1 from samples and HEBP1-HRP conjugate compete for the anti-HEBP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by HEBP1 from the sample, fewer sites are left to bind HEBP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HEBP1 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: HEBP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-HEBP1 antibody and an HEBP1-HRP conjugate. The assay sample and buffer are incubated together with HEBP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HEBP1 concentration since HEBP1 from samples and HEBP1-HRP conjugate compete for the anti-HEBP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by HEBP1 from the sample, fewer sites are left to bind HEBP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HEBP1 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for HEBP1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,097 Da
NCBI Official Full Name
heme-binding protein 1
NCBI Official Synonym Full Names
heme binding protein 1
NCBI Official Symbol
HEBP1
NCBI Official Synonym Symbols
HBP; HEBP
NCBI Protein Information
heme-binding protein 1; p22HBP
UniProt Protein Name
Heme-binding protein 1
Protein Family
UniProt Gene Name
HEBP1
UniProt Synonym Gene Names
HBP
UniProt Entry Name
HEBP1_HUMAN
NCBI Description
The full-length protein encoded by this gene is an intracellular tetrapyrrole-binding protein. This protein includes a natural chemoattractant peptide of 21 amino acids at the N-terminus, which is a natural ligand for formyl peptide receptor-like receptor 2 (FPRL2) and promotes calcium mobilization and chemotaxis in monocytes and dendritic cells. [provided by RefSeq, Jul 2008]
Uniprot Description
HEBP1: May bind free porphyrinogens that may be present in the cell and thus facilitate removal of these potentially toxic compound. Binds with a high affinity to one molecule of heme or porphyrins. It binds metalloporphyrins, free porphyrins and N- methylprotoporphyrin with similar affinities. Belongs to the HEBP family.
Chromosomal Location of Human Ortholog: 12p13.1
Cellular Component: mitochondrion; extracellular region; cytosol
Molecular Function: heme binding
Biological Process: circadian rhythm
Research Articles on HEBP1
Similar Products
Product Notes
The Mouse HEBP1 hebp1 (Catalog #AAA7245897) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7245897 ELISA Kit recognizes Mouse HEBP1. It is sometimes possible for the material contained within the vial of "Heme-binding protein 1 (HEBP1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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